Literature DB >> 3297035

Purification and kinetic properties of pyruvate kinase isoenzymes of Salmonella typhimurium.

C Garcia-Olalla, A Garrido-Pertierra.   

Abstract

Two forms of pyruvate kinase (ATP: pyruvate 2-O-phosphotransferase, EC 2.7.1.40) present in Salmonella typhimurium were purified to homogeneity from the same cultures by (NH4)2SO4 fractionation and gel filtration, anion-exchange and affinity chromatography. Mr values, subunit structure, amino acid composition and activity and stability conditions were determined for the two forms. Kinetic and regulatory properties of the two purified isoenzymes were studied.

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Year:  1987        PMID： 3297035      PMCID： PMC1147599          DOI： 10.1042/bj2410573

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  39 in total

1.  Spectroscopic determination of tryptophan and tyrosine in proteins.

Authors:  H Edelhoch
Journal:  Biochemistry       Date:  1967-07       Impact factor: 3.162

2.  The control of pyruvate kinases of Escherichia coli. I. Physicochemical and regulatory properties of the enzyme activated by fructose 1,6-diphosphate.

Authors:  E B Waygood; B D Sanwal
Journal:  J Biol Chem       Date:  1974-01-10       Impact factor: 5.157

3.  Regulation of the TCA and glyoxylate cycles in Brevibacterium flavum. II. Regulation of phosphoenolpyruvate carboxylase and pyruvate kinase.

Authors:  H Ozaki; I Shiio
Journal:  J Biochem       Date:  1969-09       Impact factor: 3.387

4.  A new convenient method for estimation of total cystine-cysteine in proteins.

Authors:  R L Spencer; F Wold
Journal:  Anal Biochem       Date:  1969-10-15       Impact factor: 3.365

5.  Pyruvate kinase of the spore-forming bacterium, Bacillus licheniformis. II. Kinetic properties.

Authors:  F W Tuominen; R W Bernlohr
Journal:  J Biol Chem       Date:  1971-03-25       Impact factor: 5.157

6.  Subunit structure of rabbit muscle pyruvate kinase.

Authors:  G L Cottam; P F Hollenberg; M J Coon
Journal:  J Biol Chem       Date:  1969-03-25       Impact factor: 5.157

7.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  K Weber; M Osborn
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157

8.  Yeast pyruvate kinase. II. Kinetic properties.

Authors:  J R Hunsley; C H Suelter
Journal:  J Biol Chem       Date:  1969-09-25       Impact factor: 5.157

9.  Factors afecting the activity of pyruvate kinase of Acetobacter xylinum.

Authors:  M Benziman
Journal:  Biochem J       Date:  1969-05       Impact factor: 3.857

10.  Glycerol kinase, the pacemaker for the dissimilation of glycerol in Escherichia coli.

Authors:  N Zwaig; W S Kistler; E C Lin
Journal:  J Bacteriol       Date:  1970-06       Impact factor: 3.490
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  6 in total

Review 1.  An overview of structure, function, and regulation of pyruvate kinases.

Authors:  Norbert Schormann; Katherine L Hayden; Paul Lee; Surajit Banerjee; Debasish Chattopadhyay
Journal:  Protein Sci       Date:  2019-08-12       Impact factor: 6.725

2.  Evolutionary plasticity in the allosteric regulator-binding site of pyruvate kinase isoform PykA from Pseudomonas aeruginosa.

Authors:  Yassmin Abdelhamid; Paul Brear; Jack Greenhalgh; Xavier Chee; Taufiq Rahman; Martin Welch
Journal:  J Biol Chem       Date:  2019-09-04       Impact factor: 5.157

3.  The Manganese-Dependent Pyruvate Kinase PykM Is Required for Wild-Type Glucose Utilization by Brucella abortus 2308 and Its Virulence in C57BL/6 Mice.

Authors:  Joshua E Pitzer; Tonya N Zeczycki; John E Baumgartner; Daniel W Martin; R Martin Roop
Journal:  J Bacteriol       Date:  2018-11-26       Impact factor: 3.490

4.  Structural and functional analysis of pyruvate kinase from Corynebacterium glutamicum.

Authors:  M S Jetten; M E Gubler; S H Lee; A J Sinskey
Journal:  Appl Environ Microbiol       Date:  1994-07       Impact factor: 4.792

5.  The contribution of two isozymes to the pyruvate kinase activity of Vibrio cholerae: One K+-dependent constitutively active and another K+-independent with essential allosteric activation.

Authors:  Carlos Guerrero-Mendiola; José J García-Trejo; Rusely Encalada; Emma Saavedra; Leticia Ramírez-Silva
Journal:  PLoS One       Date:  2017-07-07       Impact factor: 3.240

6.  Allosteric pyruvate kinase-based "logic gate" synergistically senses energy and sugar levels in Mycobacterium tuberculosis.

Authors:  Wenhe Zhong; Liang Cui; Boon Chong Goh; Qixu Cai; Peiying Ho; Yok Hian Chionh; Meng Yuan; Abbas El Sahili; Linda A Fothergill-Gilmore; Malcolm D Walkinshaw; Julien Lescar; Peter C Dedon
Journal:  Nat Commun       Date:  2017-12-07       Impact factor: 14.919
  6 in total

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