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Literature DB >> 386280

Specific fluorescent labeling of 7-(aminomethyl)-7-deazaguanosine located in the anticodon of tRNATyr isolated from E. coli mutant.

H Kasai, N Shindo-Okada, S Noguchi, S Nishimura.

Abstract

Under-modified E. coli tRNATyr that contains 7-(aminomethyl)-7-deazaguanosine in place of Q nucleoside can be chemically modified by dansyl chloride under neutral conditions. Fluorescent labelling specifically occurred only in the 7-(aminomethyl)-7-deazaguanine moiety. The modified tRNATyr was found to be active both in aminoacylation and in binding to ribosomes.

Entities: Chemical Species

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Year: 1979 PMID： 386280 PMCID： PMC328008 DOI： 10.1093/nar/7.1.231

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

12 in total

1. Replacement of Y base, dihydrouracil, and 7-methylguanine in tRNA by artificial odd bases.

Authors: W Wintermeyer; H G. Zachau
Journal: FEBS Lett Date: 1971-11-01 Impact factor: 4.124

2. Resistance of S-RNA to ribonucleases in the presence of magnesium ion.

Authors: S NISHIMURA; G D NOVELLI
Journal: Biochem Biophys Res Commun Date: 1963-05-03 Impact factor: 3.575

3. Structure determination of a nucleoside Q precursor isolated from E. coli tRNA: 7-(aminomethyl)-7-deazaguanosine.

Authors: N Okada; S Noguchi; S Nishimura; T Ohgi; T Goto; P F Crain; J A McCloskey
Journal: Nucleic Acids Res Date: 1978-07 Impact factor: 16.971

4. Nature of magnesium-induced miscoding.

Authors: S Nishimura; F Harada; M Hirabayashi
Journal: J Mol Biol Date: 1969-03-14 Impact factor: 5.469

5. Studies on polynucleotides. 48. The in vitro synthesis of a co-polypeptide containing two amino acids in alternating sequence dependent upon a DNA-like polymer containing two nucleotides in alternating sequence.

Authors: S Nishimura; D S Jones; H G Khorana
Journal: J Mol Biol Date: 1965-08 Impact factor: 5.469

6. Studies on the conformation of the anticodon loop of phenylalanine transfer ribonucleic acid. Effect of environment on the fluorescence of the Y base.

Authors: K Beardsley; T Tao; C R Cantor
Journal: Biochemistry Date: 1970-09-01 Impact factor: 3.162

7. Purification of leucyl transfer ribonucleic acid synthetase from Escherichia coli.

Authors: H Hayashi; J R Knowles; J R Katze; J Lapointe; D Söll
Journal: J Biol Chem Date: 1970-03-25 Impact factor: 5.157

8. Novel mechanism of post-transcriptional modification of tRNA. Insertion of bases of Q precursors into tRNA by a specific tRNA transglycosylase reaction.

Authors: N Okada; S Noguchi; H Kasai; N Shindo-Okada; T Ohgi; T Goto; S Nishimura
Journal: J Biol Chem Date: 1979-04-25 Impact factor: 5.157

Specific fluorescent labeling of 7-(aminomethyl)-7-deazaguanosine located in the anticodon of tRNATyr isolated from E. coli mutant.

1. Replacement of Y base, dihydrouracil, and 7-methylguanine in tRNA by artificial odd bases.

2. Resistance of S-RNA to ribonucleases in the presence of magnesium ion.

3. Structure determination of a nucleoside Q precursor isolated from E. coli tRNA: 7-(aminomethyl)-7-deazaguanosine.

4. Nature of magnesium-induced miscoding.

5. Studies on polynucleotides. 48. The in vitro synthesis of a co-polypeptide containing two amino acids in alternating sequence dependent upon a DNA-like polymer containing two nucleotides in alternating sequence.

6. Studies on the conformation of the anticodon loop of phenylalanine transfer ribonucleic acid. Effect of environment on the fluorescence of the Y base.

7. Purification of leucyl transfer ribonucleic acid synthetase from Escherichia coli.

8. Novel mechanism of post-transcriptional modification of tRNA. Insertion of bases of Q precursors into tRNA by a specific tRNA transglycosylase reaction.

9. Studies of transfer RNA tertiary structure of singlet-singlet energy transfer.

10. Studies of transfer RNA tertiary structure by singlet-singlet energy transfer.

1. Use of specific chemical reagents for detection of modified nucleotides in RNA.

2. A multifunctional bioconjugate module for versatile photoaffinity labeling and click chemistry of RNA.

3. Structure-function relationship of substituted bromomethylcoumarins in nucleoside specificity of RNA alkylation.