Literature DB >> 3859867

Isolation and characterization of two fractions from HeLa cells required for mRNA splicing in vitro.

H M Furneaux, K K Perkins, G A Freyer, J Arenas, J Hurwitz.   

Abstract

A nuclear extract from HeLa cells has been separated by DEAE-cellulose chromatography into two fractions, both of which are required for mRNA splicing in vitro. Both fractions are heat labile and sensitive to N-ethylmaleimide. The activity of one of the fractions was abolished by preincubation with micrococcal nuclease, while the other fraction was unaffected by this treatment. This abolition indicates an essential nucleic acid component. Fractions I and II are required for the in vitro splicing of human beta-globin and adenovirus transcripts.

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Year:  1985        PMID： 3859867      PMCID： PMC390411          DOI： 10.1073/pnas.82.13.4351

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  14 in total

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Authors:  J Abelson
Journal:  Annu Rev Biochem       Date:  1979       Impact factor: 23.643

2.  Splicing of in vitro synthesized messenger RNA precursors in HeLa cell extracts.

Authors:  N Hernandez; W Keller
Journal:  Cell       Date:  1983-11       Impact factor: 41.582

3.  Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein.

Authors:  R A Padgett; S M Mount; J A Steitz; P A Sharp
Journal:  Cell       Date:  1983-11       Impact factor: 41.582

4.  The U1 small nuclear RNA-protein complex selectively binds a 5' splice site in vitro.

Authors:  S M Mount; I Pettersson; M Hinterberger; A Karmas; J A Steitz
Journal:  Cell       Date:  1983-06       Impact factor: 41.582

5.  The orderly splicing of the first three leaders of the adenovirus-2 major late transcript.

Authors:  P Keohavong; R Gattoni; J M LeMoullec; M Jacob; J Stévenin
Journal:  Nucleic Acids Res       Date:  1982-02-25       Impact factor: 16.971

6.  Are snRNPs involved in splicing?

Authors:  M R Lerner; J A Boyle; S M Mount; S L Wolin; J A Steitz
Journal:  Nature       Date:  1980-01-10       Impact factor: 49.962

7.  Bacteriophage SP6-specific RNA polymerase. I. Isolation and characterization of the enzyme.

Authors:  E T Butler; M J Chamberlin
Journal:  J Biol Chem       Date:  1982-05-25       Impact factor: 5.157

8.  Cofactor requirements of splicing of purified messenger RNA precursors.

Authors:  S F Hardy; P J Grabowski; R A Padgett; P A Sharp
Journal:  Nature       Date:  1984 Mar 22-28       Impact factor: 49.962

9.  Normal and mutant human beta-globin pre-mRNAs are faithfully and efficiently spliced in vitro.

Authors:  A R Krainer; T Maniatis; B Ruskin; M R Green
Journal:  Cell       Date:  1984-04       Impact factor: 41.582

10.  Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.

Authors:  J D Dignam; R M Lebovitz; R G Roeder
Journal:  Nucleic Acids Res       Date:  1983-03-11       Impact factor: 16.971
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  15 in total

1.  The identification of an endonuclease that cleaves within an HuR binding site in mRNA.

Authors:  Z Zhao; F C Chang; H M Furneaux
Journal:  Nucleic Acids Res       Date:  2000-07-15       Impact factor: 16.971

2.  A block in mammalian splicing occurring after formation of large complexes containing U1, U2, U4, U5, and U6 small nuclear ribonucleoproteins.

Authors:  C H Agris; M E Nemeroff; R M Krug
Journal:  Mol Cell Biol       Date:  1989-01       Impact factor: 4.272

3.  Alterations of RNase H sensitivity of the 3' splice site region during the in vitro splicing reaction.

Authors:  H Sawa; Y Shimura
Journal:  Nucleic Acids Res       Date:  1991-07-25       Impact factor: 16.971

4.  Pre-mRNA splicing and the nuclear matrix.

Authors:  S Zeitlin; A Parent; S Silverstein; A Efstratiadis
Journal:  Mol Cell Biol       Date:  1987-01       Impact factor: 4.272

5.  Requirements for accurate and efficient mRNA 3' end cleavage and polyadenylation of a simian virus 40 early pre-RNA in vitro.

Authors:  L C Ryner; J L Manley
Journal:  Mol Cell Biol       Date:  1987-01       Impact factor: 4.272

6.  RNA splicing products formed with isolated fractions from HeLa cells are associated with fast-sedimenting complexes.

Authors:  K K Perkins; H M Furneaux; J Hurwitz
Journal:  Proc Natl Acad Sci U S A       Date:  1986-02       Impact factor: 11.205

7.  Fractionation and characterization of a yeast mRNA splicing extract.

Authors:  S C Cheng; J Abelson
Journal:  Proc Natl Acad Sci U S A       Date:  1986-04       Impact factor: 11.205

8.  Three distinct activities possibly involved in mRNA splicing are found in a nuclear fraction lacking U1 and U2 RNA.

Authors:  A Mayeda; K Tatei; H Kitayama; K Takemura; Y Ohshima
Journal:  Nucleic Acids Res       Date:  1986-04-11       Impact factor: 16.971

9.  In vitro splicing of pre-messenger RNA with extracts from 5-fluorouridine-treated cells.

Authors:  J R Patton
Journal:  Biochem J       Date:  1994-01-15       Impact factor: 3.857

10.  An ordered pathway of snRNP binding during mammalian pre-mRNA splicing complex assembly.

Authors:  A Bindereif; M R Green
Journal:  EMBO J       Date:  1987-08       Impact factor: 11.598
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