Literature DB >> 3856873

Characterization of a yeast mitochondrial promoter by deletion mutagenesis.

T K Biswas, J C Edwards, M Rabinowitz, G S Getz.   

Abstract

We have generated collections of mutants of the promoter for the small rRNA gene from the mitochondria of yeast deleted from either the 3' or 5' end. Plasmids containing the partially deleted promoter were assayed for their ability to direct correct transcriptional initiation in a homologous in vitro system. We find that the region required for high-efficiency promoter function lies between positions -10 and +2. Our methods detected no effect of flanking sequences on the strength of this promoter.

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Year:  1985        PMID： 3856873      PMCID： PMC397457          DOI： 10.1073/pnas.82.7.1954

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  22 in total

Review 1.  Regulatory sequences involved in the promotion and termination of RNA transcription.

Authors:  M Rosenberg; D Court
Journal:  Annu Rev Genet       Date:  1979       Impact factor: 16.830

2.  pUR222, a vector for cloning and rapid chemical sequencing of DNA.

Authors:  U Rüther; M Koenen; K Otto; B Müller-Hill
Journal:  Nucleic Acids Res       Date:  1981-08-25       Impact factor: 16.971

3.  Transcriptional initiation and 5' termini of yeast mitochondrial RNA.

Authors:  D Levens; B Ticho; E Ackerman; M Rabinowitz
Journal:  J Biol Chem       Date:  1981-05-25       Impact factor: 5.157

4.  pUR 250 allows rapid chemical sequencing of both DNA strands of its inserts.

Authors:  U Rüther
Journal:  Nucleic Acids Res       Date:  1982-10-11       Impact factor: 16.971

5.  Transcriptional initiation and processing of the small ribosomal RNA of yeast mitochondria.

Authors:  T Christianson; J Edwards; D Levens; J Locker; M Rabinowitz
Journal:  J Biol Chem       Date:  1982-06-10       Impact factor: 5.157

6.  Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors:  A M Maxam; W Gilbert
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

7.  A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.

Authors:  D F Bogenhagen; S Sakonju; D D Brown
Journal:  Cell       Date:  1980-01       Impact factor: 41.582

8.  A set of synthetic oligodeoxyribonucleotide primers for DNA sequencing in the plasmid vector pBR322.

Authors:  R B Wallace; M J Johnson; S V Suggs; K Miyoshi; R Bhatt; K Itakura
Journal:  Gene       Date:  1981-12       Impact factor: 3.688

9.  Mitochondrial transcription complex from Saccharomyces cerevisiae.

Authors:  D Levens; R Morimoto; M Rabinowitz
Journal:  J Biol Chem       Date:  1981-02-10       Impact factor: 5.157

10.  Purification of mitochondrial RNA polymerase from Saccharomyces cerevisiae.

Authors:  D Levens; A Lustig; M Rabinowitz
Journal:  J Biol Chem       Date:  1981-02-10       Impact factor: 5.157
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  35 in total

1.  Sugarbeet minicircular mitochondrial DNAs: high-resolution transcript mapping, transcript abundance and copy number determination.

Authors:  C M Thomas
Journal:  Mol Gen Genet       Date:  1992-09

2.  Characterization of transcription initiation sites on the soybean mitochondrial genome allows identification of a transcription-associated sequence motif.

Authors:  G G Brown; A H Auchincloss; P S Covello; M W Gray; R Menassa; M Singh
Journal:  Mol Gen Genet       Date:  1991-09

3.  Transcription and RNA-processing in fission yeast mitochondria.

Authors:  Bernd Schäfer; Monika Hansen; B Franz Lang
Journal:  RNA       Date:  2005-04-05       Impact factor: 4.942

4.  Mitochondrial transcription factor Mtf1 traps the unwound non-template strand to facilitate open complex formation.

Authors:  Swaroopa Paratkar; Smita S Patel
Journal:  J Biol Chem       Date:  2009-12-11       Impact factor: 5.157

5.  Novel method for identifying sequence-specific DNA-binding proteins.

Authors:  D Levens; P M Howley
Journal:  Mol Cell Biol       Date:  1985-09       Impact factor: 4.272

6.  Precise assignment of the light-strand promoter of mouse mitochondrial DNA: a functional promoter consists of multiple upstream domains.

Authors:  D D Chang; D A Clayton
Journal:  Mol Cell Biol       Date:  1986-09       Impact factor: 4.272

7.  Identification of a new promoter within the tRNA gene cluster of the mitochondrial DNA of Saccharomyces cerevisiae.

Authors:  J S Backer; G S Getz
Journal:  Nucleic Acids Res       Date:  1987-11-25       Impact factor: 16.971

8.  Nucleotides flanking the promoter sequence influence the transcription of the yeast mitochondrial gene coding for ATPase subunit 9.

Authors:  T K Biswas; G S Getz
Journal:  Proc Natl Acad Sci U S A       Date:  1986-01       Impact factor: 11.205

9.  Effect of point mutations on in vitro transcription from the promoter for the large ribosomal RNA gene of yeast mitochondria.

Authors:  A H Schinkel; M J Groot Koerkamp; M H Stuiver; G T Van der Horst; H F Tabak
Journal:  Nucleic Acids Res       Date:  1987-07-24       Impact factor: 16.971

10.  Identification of transcriptional regulatory elements in human mitochondrial DNA by linker substitution analysis.

Authors:  J N Topper; D A Clayton
Journal:  Mol Cell Biol       Date:  1989-03       Impact factor: 4.272
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