Literature DB >> 3856845

Stabilization of a primary loop in myosin subfragment 1 with a fluorescent crosslinker.

D Mornet, K Ue, M F Morales.   

Abstract

A bifunctional fluorescent alkylating agent, dibromobimane, has been used to stabilize a preexisting primary loop in myosin subfragment 1 (S-1). The crosslink achieved joins Cys-707 (called sulfhydryl group "SH1") of the 20-kDa domain (formerly called "20K" domain) with a thiol of the 50-kDa domain and seems to place the dibromobimane near the ATP-perturbable tryptophan.

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Year:  1985        PMID： 3856845      PMCID： PMC397331          DOI： 10.1073/pnas.82.6.1658

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  27 in total

1.  Separation of subfragment-1 isoenzymes from rabbit skeletal muscle myosin.

Authors:  A G Weeds; R S Taylor
Journal:  Nature       Date:  1975-09-04       Impact factor: 49.962

2.  Location of SH-1 and SH-2 in the heavy chain segment of heavy meromyosin.

Authors:  M Bálint; I Wolf; A Tarcsafalvi; J Gergely; F A Sréter
Journal:  Arch Biochem Biophys       Date:  1978-10       Impact factor: 4.013

3.  Detection of conformational changes in actin by proteolytic digestion: evidence for a new monomeric species.

Authors:  S A Rich; J E Estes
Journal:  J Mol Biol       Date:  1976-07-15       Impact factor: 5.469

4.  Cleavage at Asn-Gly bonds with hydroxylamine.

Authors:  P Bornstein; G Balian
Journal:  Methods Enzymol       Date:  1977       Impact factor: 1.600

5.  Troponin-tropomyosin complex. Column chromatographic separation and activity of the three, active troponin components with and without tropomyosin present.

Authors:  E Eisenberg; W W Kielley
Journal:  J Biol Chem       Date:  1974-08-10       Impact factor: 5.157

6.  A new protein of the thick filaments of vertebrate skeletal myofibrils. Extractions, purification and characterization.

Authors:  G Offer; C Moos; R Starr
Journal:  J Mol Biol       Date:  1973-03-15       Impact factor: 5.469

7.  Substructure of the myosin molecule. I. Subfragments of myosin by enzymic degradation.

Authors:  S Lowey; H S Slayter; A G Weeds; H Baker
Journal:  J Mol Biol       Date:  1969-05-28       Impact factor: 5.469

8.  Analysis of bacteriophage T7 early RNAs and proteins on slab gels.

Authors:  F W Studier
Journal:  J Mol Biol       Date:  1973-09-15       Impact factor: 5.469

9.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

10.  Heterogeneity of myosin heavy chains in subfragment-1 isoenzymes rabbit skeletal myosin.

Authors:  B J Pope; P D Wagner; A G Weeds
Journal:  J Mol Biol       Date:  1977-01-25       Impact factor: 5.469
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  10 in total

1.  Actin and temperature effects on the cross-linking of the SH1-SH2 helix in myosin subfragment 1.

Authors:  L K Nitao; E Reisler
Journal:  Biophys J       Date:  2000-06       Impact factor: 4.033

2.  Domain rearrangement of SRP protein Ffh upon binding 4.5S RNA and the SRP receptor FtsY.

Authors:  Iwona Buskiewicz; Andriy Kubarenko; Frank Peske; Marina V Rodnina; Wolfgang Wintermeyer
Journal:  RNA       Date:  2005-06       Impact factor: 4.942

3.  A search for protein structural changes accompanying the contractile interaction.

Authors:  W C Johnson; D B Bivin; K Ue; M F Morales
Journal:  Proc Natl Acad Sci U S A       Date:  1991-11-01       Impact factor: 11.205

Review 4.  Domains, motions and regulation in the myosin head.

Authors:  P Vibert; C Cohen
Journal:  J Muscle Res Cell Motil       Date:  1988-08       Impact factor: 2.698

5.  On the origin and transmission of force in actomyosin subfragment 1.

Authors:  J Botts; J F Thomason; M F Morales
Journal:  Proc Natl Acad Sci U S A       Date:  1989-04       Impact factor: 11.205

Review 6.  Pathway for the communication between the ATPase and actin sites in myosin.

Authors:  E Audemard; R Bertrand; A Bonet; P Chaussepied; D Mornet
Journal:  J Muscle Res Cell Motil       Date:  1988-06       Impact factor: 2.698

7.  Consequences of placing an intramolecular crosslink in myosin S1.

Authors:  K Konno; K Ue; M Khoroshev; H Martinez; B Ray; M F Morales
Journal:  Proc Natl Acad Sci U S A       Date:  2000-02-15       Impact factor: 11.205

8.  Nucleotide trapping at the ATPase site of myosin subfragment 1 by a new interthiol crosslinking.

Authors:  P Chaussepied; D Mornet; R Kassab
Journal:  Proc Natl Acad Sci U S A       Date:  1986-04       Impact factor: 11.205

9.  Both the 25-kDa and 50-kDa domains in myosin subfragment 1 are close to the reactive thiols.

Authors:  R C Lu; L Moo; A G Wong
Journal:  Proc Natl Acad Sci U S A       Date:  1986-09       Impact factor: 11.205

Review 10.  Functional sequences of the myosin head.

Authors:  D Mornet; A Bonet; E Audemard; J Bonicel
Journal:  J Muscle Res Cell Motil       Date:  1989-02       Impact factor: 2.698
  10 in total

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