Literature DB >> 3839937

Molecular cloning of complementary DNA for the alpha subunit of the G protein that stimulates adenylate cyclase.

B A Harris, J D Robishaw, S M Mumby, A G Gilman.   

Abstract

A complementary DNA clone encoding the alpha subunit of the adenylate cyclase stimulatory G protein (Gs) was isolated and identified. A bovine brain complementary DNA library was screened with an oligonucleotide probe derived from amino acid sequence common to known G proteins. The only clone that was obtained with this probe has a complementary DNA insert of approximately 1670 base pairs. An antibody to a peptide synthesized according to deduced amino acid sequence reacts specifically with the alpha subunit of Gs. In addition, RNA that hybridizes with probes made from the clone is detected in wild-type S49 cells; however, cyc- S49 cells, which are deficient in Gs alpha activity, are devoid of this messenger RNA.

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Year:  1985        PMID： 3839937     DOI： 10.1126/science.3839937

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  39 in total

1.  Genetic deficiency of the alpha subunit of the guanine nucleotide-binding protein Gs as the molecular basis for Albright hereditary osteodystrophy.

Authors:  M A Levine; T G Ahn; S F Klupt; K D Kaufman; P M Smallwood; H R Bourne; K A Sullivan; C Van Dop
Journal:  Proc Natl Acad Sci U S A       Date:  1988-01       Impact factor: 11.205

2.  Mutation of the Gs protein alpha subunit NH2 terminus relieves an attenuator function, resulting in constitutive adenylyl cyclase stimulation.

Authors:  S Osawa; L E Heasley; N Dhanasekaran; S K Gupta; C W Woon; C Berlot; G L Johnson
Journal:  Mol Cell Biol       Date:  1990-06       Impact factor: 4.272

3.  Preservation of insulin mRNA levels and insulin secretion in HIT cells by avoidance of chronic exposure to high glucose concentrations.

Authors:  R P Robertson; H J Zhang; K L Pyzdrowski; T F Walseth
Journal:  J Clin Invest       Date:  1992-08       Impact factor: 14.808

4.  RGS3 controls T lymphocyte migration in a model of Th2-mediated airway inflammation.

Authors:  Jesse W Williams; Douglas Yau; Nan Sethakorn; Jacob Kach; Eleanor B Reed; Tamson V Moore; Judy Cannon; Xiaohua Jin; Heming Xing; Anthony J Muslin; Anne I Sperling; Nickolai O Dulin
Journal:  Am J Physiol Lung Cell Mol Physiol       Date:  2013-09-27       Impact factor: 5.464

5.  Density-dependent nerve growth factor regulation of Gs-alpha RNA in pheochromocytoma 12 cells.

Authors:  G Tjaden; A Aguanno; R Kumar; D Benincasa; R M Gubits; H Yu; K P Dolan
Journal:  Mol Cell Biol       Date:  1990-06       Impact factor: 4.272

6.  Stimulation of beta-adrenergic receptors of S49 lymphoma cells redistributes the alpha subunit of the stimulatory G protein between cytosol and membranes.

Authors:  L A Ransnäs; P Svoboda; J R Jasper; P A Insel
Journal:  Proc Natl Acad Sci U S A       Date:  1989-10       Impact factor: 11.205

7.  Deduced primary structure of the alpha subunit of the GTP-binding stimulatory protein of adenylate cyclase.

Authors:  J D Robishaw; D W Russell; B A Harris; M D Smigel; A G Gilman
Journal:  Proc Natl Acad Sci U S A       Date:  1986-03       Impact factor: 11.205

8.  Molecular cloning and sequence determination of cDNAs for alpha subunits of the guanine nucleotide-binding proteins Gs, Gi, and Go from rat brain.

Authors:  H Itoh; T Kozasa; S Nagata; S Nakamura; T Katada; M Ui; S Iwai; E Ohtsuka; H Kawasaki; K Suzuki
Journal:  Proc Natl Acad Sci U S A       Date:  1986-06       Impact factor: 11.205

9.  Drosophila stimulatory G protein alpha subunit activates mammalian adenylyl cyclase but interacts poorly with mammalian receptors: implications for receptor-G protein interaction.

Authors:  F Quan; L Thomas; M Forte
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-01       Impact factor: 11.205

10.  Oxytocin receptor couples to the 80 kDa Gh alpha family protein in human myometrium.

Authors:  K J Baek; N S Kwon; H S Lee; M S Kim; P Muralidhar; M J Im
Journal:  Biochem J       Date:  1996-05-01       Impact factor: 3.857
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