Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Chicken erythrocyte polynucleosomes which are soluble at physiological ionic strength and contain linker histones are highly enriched in beta-globin gene sequences.

Literature DB >> 3822820

Chicken erythrocyte polynucleosomes which are soluble at physiological ionic strength and contain linker histones are highly enriched in beta-globin gene sequences.

Abstract

Mature chicken erythrocyte polynucleosomes which are soluble at physiological ionic strength are enriched in beta-globin DNA sequences. Vitellogenin chromatin, which is not expressed in this tissue, is found in aggregation prone, salt insoluble chromatin. There is a direct correlation between the size of soluble fragments and the degree of globin gene enrichment, with the largest fragments being most highly enriched. The highly globin enriched (about 50 fold) polynucleosomes contain significantly elevated levels of acetylated histones H4, H2A.Z, and H2B, and ubiquitinated (prefix "u") histones H2A and H2B (with a significant relative increase of uH2B over uH2A). These polynucleosomes were complexed with histones H1 and H5 but at a lower level than that found in unfractionated chromatin.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1987 PMID： 3822820 PMCID： PMC340509 DOI： 10.1093/nar/15.3.1081

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

27 in total

1. The nonhistone chromosomal protein, H2A-specific protease, is selectively associated with nucleosomes containing histone H1.

Authors: J R Davie; L Numerow; G P Delcuve
Journal: J Biol Chem Date: 1986-08-05 Impact factor: 5.157

9. Formaldehyde cross-linking and immunoprecipitation demonstrate developmental changes in H1 association with transcriptionally active genes.

Authors: P C Dedon; J A Soults; C D Allis; M A Gorovsky
Journal: Mol Cell Biol Date: 1991-03 Impact factor: 4.272

10. Nucleosomal histones of transcriptionally active/competent chromatin preferentially exchange with newly synthesized histones in quiescent chicken erythrocytes.

Authors: M J Hendzel; J R Davie
Journal: Biochem J Date: 1990-10-01 Impact factor: 3.857

Chicken erythrocyte polynucleosomes which are soluble at physiological ionic strength and contain linker histones are highly enriched in beta-globin gene sequences.

1. The nonhistone chromosomal protein, H2A-specific protease, is selectively associated with nucleosomes containing histone H1.

2. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

3. Metabolism of ubiquitinated histones.

4. Rapid turnover of the histone-ubiquitin conjugate, protein A24.

5. The effect of histone hyperacetylation on the nuclease sensitivity and the solubility of chromatin.

6. Two-dimensional gel systems for rapid histone analysis for use in minislab polyacrylamide gel electrophoresis.

7. Only a small fraction of avian erythrocyte histone is involved in ongoing acetylation.

8. Exchange of histone H1 between segments of chromatin.

9. Metabolism of histones in avian erythroid cells.

10. Quantitative determination of histone modification. H2A acetylation and phosphorylation.

1. Purification and initial characterization of primate satellite chromatin.

2. Analysis of erythroid nuclear proteins binding to the promoter and enhancer elements of the chicken histone H5 gene.

3. Histone acetylation and globin gene switching.

4. Loosened nucleosome linker folding in transcriptionally active chromatin of chicken embryo erythrocyte nuclei.

5. Ultrastructure of transcriptionally competent chromatin.

6. Nucleoprotein hybridization: a method for isolating active and inactive genes as chromatin.

7. Multisubunit erythroid complexes binding to the enhancer element of the chicken histone H5 gene.

Review 8. On the biological role of histone acetylation.

9. Formaldehyde cross-linking and immunoprecipitation demonstrate developmental changes in H1 association with transcriptionally active genes.

10. Nucleosomal histones of transcriptionally active/competent chromatin preferentially exchange with newly synthesized histones in quiescent chicken erythrocytes.