Literature DB >> 3814086

A high-performance-liquid-chromatographic method for the assay of coproporphyrinogen oxidase activity in rat liver.

F Li, C K Lim, T J Peters.   

Abstract

An h.p.l.c. method was developed for the assay of coproporphyrinogen oxidase activity in rat liver. The protoporphyrinogen IX formed is completely oxidized to protoporphyrin IX for separation and quantification by reversed-phase chromatography with mesoporphyrin as the internal standard. The Km of coproporphrinogen oxidase is 1.01 +/- 0.23 microM. The activities are 4.07 +/- 0.40 nmol of protoporphyrin IX/h per mg of mitochondrial protein and 224 +/- 19 nmol of protoporphyrin IX/h per g of liver tissue homogenate. The method is sensitive enough for measuring enzyme activity in small amounts of human tissue from needle biopsy.

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Year:  1986        PMID： 3814086      PMCID： PMC1147306          DOI： 10.1042/bj2390481

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  9 in total

1.  Mitochondrial coproporphyrinogen oxidase and protoporphyrin formation.

Authors:  S SANO; S GRANICK
Journal:  J Biol Chem       Date:  1961-04       Impact factor: 5.157

2.  A simplified method for the quantitative assay of small amounts of protein in biologic material.

Authors:  G R Schacterle; R L Pollack
Journal:  Anal Biochem       Date:  1973-02       Impact factor: 3.365

3.  The direct linear plot. A new graphical procedure for estimating enzyme kinetic parameters.

Authors:  R Eisenthal; A Cornish-Bowden
Journal:  Biochem J       Date:  1974-06       Impact factor: 3.857

4.  Aerobic and anaerobic coproporphyrinogenase activities in extracts from Saccharomyces cerevisiae.

Authors:  R Poulson; W J Polglase
Journal:  J Biol Chem       Date:  1974-10-25       Impact factor: 5.157

5.  Coproporphyrinogen III oxidase assay.

Authors:  B Grandchamp; Y Nordmann
Journal:  Enzyme       Date:  1982

6.  Evidence that the coproporphyrinogen oxidase activity of rat liver is situated in the intermembrane space of mitochondria.

Authors:  G H Elder; J O Evans
Journal:  Biochem J       Date:  1978-05-15       Impact factor: 3.857

7.  Coproporphyrinogenase activities in extracts of Rhodopseudomonas spheroides and Chromatium strain D.

Authors:  G H Tait
Journal:  Biochem J       Date:  1972-08       Impact factor: 3.857

8.  A radiochemical method for the measurement of coproporphyrinogen oxidase and the utilization of substrates other than coproporphyrinogen III by the enzyme from rat liver.

Authors:  G H Elder; J O Evans
Journal:  Biochem J       Date:  1978-01-01       Impact factor: 3.857

9.  Purification and properties of coproporphyrinogenase.

Authors:  A M del Batlle; A Benson; C Rimington
Journal:  Biochem J       Date:  1965-12       Impact factor: 3.857
  9 in total
  2 in total

1.  An h.p.l.c. assay for protoporphyrinogen oxidase activity in rat liver.

Authors:  F Li; C K Lim; T J Peters
Journal:  Biochem J       Date:  1987-05-01       Impact factor: 3.857

2.  Direct assay of enzymes in heme biosynthesis for the detection of porphyrias by tandem mass spectrometry. Uroporphyrinogen decarboxylase and coproporphyrinogen III oxidase.

Authors:  Yuesong Wang; Paula Gatti; Martin Sadílek; C Ronald Scott; Frantisek Turecek; Michael H Gelb
Journal:  Anal Chem       Date:  2008-02-23       Impact factor: 6.986
  2 in total

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