Literature DB >> 3805735

Production and characterization of monoclonal antibodies to human tumor necrosis factor.

M Hirai, N Okamura, Y Terano, M Tsujimoto, H Nakazato.   

Abstract

Five stable hybridoma cell lines secreting antibodies to recombinant human tumor necrosis factor (TNF) were established. All monoclonal antibodies belong to the IgG1 subclass. One monoclonal antibody (MAB) (designated as 3B10) neutralizes the L929 cytotoxic activity of natural and recombinant human TNF. Other MABs bind to human TNF but do not neutralize cytotoxic activity. A sandwich enzyme immunoassay specific to human TNF molecule has been developed using 3B10. This assay measures only biologically active human TNF molecules and is as sensitive as a bioassay which measures the cytotoxic activity on L929 cells. These results show that 3B10 is quite useful in studying the biological functions of TNF.

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Year:  1987        PMID： 3805735     DOI： 10.1016/0022-1759(87)90367-x

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  8 in total

1.  Production of polyclonal antibodies to feline tumor necrosis factor.

Authors:  C M Otto; F Niagro; R A McGraw; C A Rawlings
Journal:  Clin Diagn Lab Immunol       Date:  1997-07

2.  Neutrophil activation on biological surfaces. Massive secretion of hydrogen peroxide in response to products of macrophages and lymphocytes.

Authors:  C F Nathan
Journal:  J Clin Invest       Date:  1987-12       Impact factor: 14.808

Review 3.  Pharmaceutical aspects of anti-inflammatory TNF-blocking drugs.

Authors:  Sandhya Jinesh
Journal:  Inflammopharmacology       Date:  2015-02-17       Impact factor: 4.473

4.  The endogenous induction of tumor necrosis factor serum (TNS) for the adjuvant postoperative immunotherapy of cancer--changes in immunological markers of the blood.

Authors:  Y Abe; M Miyake; T Miyazaki; A Horiuchi; S Kimura
Journal:  Jpn J Surg       Date:  1990-01

5.  Induction of the transcription factor IRF-1 and interferon-beta mRNAs by cytokines and activators of second-messenger pathways.

Authors:  T Fujita; L F Reis; N Watanabe; Y Kimura; T Taniguchi; J Vilcek
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

6.  Human tumor necrosis factor increases the resistance against Listeria infection in mice.

Authors:  K Kato; A Nakane; T Minagawa; N Kasai; K Yamamoto; N Sato; N Tsuruoka
Journal:  Med Microbiol Immunol       Date:  1989       Impact factor: 3.402

7.  Cytokine production by human colonic intraepithelial lymphocytes in controls and ulcerative colitis.

Authors:  P Hoang; H R Dalton; H J de Silv; D P Jewell
Journal:  Mediators Inflamm       Date:  1994       Impact factor: 4.711

8.  Changes and Relationship of PAF and TNF in Rats with Myocardial Ischaemia and Reperfusion Injury.

Authors:  Y L Han; S H Li; Q Y Zheng; H B Wang; G Y Zhang; Z G Wu; S C Chen
Journal:  Mediators Inflamm       Date:  1994       Impact factor: 4.711
  8 in total

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