Dynamic light scattering in the intact rabbit lens. Its relation to protein concentration.

The physical-chemical properties of highly concentrated protein systems as seen in the lens are poorly understood. Using the technique of dynamic laser light scattering spectroscopy, the authors have determined changes in the dynamics of lens proteins from the intact rabbit lens as a function of position and age. The authors have identified two heterogeneous populations of mobile scatterers with relaxation times of less than 0.5 msec and greater than 1 msec. A decrease in scattered light intensity at 90 degrees, an increase in protein concentration, and aging are associated with lengthening of the relaxation times of the less mobile population of scatterers. This data provides evidence for the existence of a gel-like structure of the lens proteins.