Literature DB >> 3801274

Cloning of mRNA sequences from the human colon: preliminary characterisation of defined mRNAs in normal and neoplastic tissues.

R A Bartsch, C Joannou, I C Talbot, D S Bailey.   

Abstract

RNA has been extracted from the normal human colon, converted into cDNA and cloned in the bacterial plasmid pBR322. About 4,000 sequences from this library were screened with probes derived from normal mucosa, familial polyposis mucosa, colonic adenocarcinomas and the colon tumour cell line HT29. Some mucosal sequences showed greatly reduced levels of transcription in neoplastic conditions, while a few showed elevated transcription. These have been further characterised by Northern and RNA dot-blot analysis.

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Year:  1986        PMID： 3801274      PMCID： PMC2001548          DOI： 10.1038/bjc.1986.242

Source DB:  PubMed          Journal:  Br J Cancer        ISSN： 0007-0920            Impact factor:   7.640


  30 in total

1.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

2.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

3.  Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

Authors:  P S Thomas
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205

4.  Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes.

Authors:  D W Cleveland; M A Lopata; R J MacDonald; N J Cowan; W J Rutter; M W Kirschner
Journal:  Cell       Date:  1980-05       Impact factor: 41.582

5.  Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.

Authors:  G K McMaster; G G Carmichael
Journal:  Proc Natl Acad Sci U S A       Date:  1977-11       Impact factor: 11.205

6.  Hormone-dependent terminal differentiation in vitro of chicken erythroleukemia cells transformed by ts mutants of avian erythroblastosis virus.

Authors:  H Beug; S Palmieri; C Freudenstein; H Zentgraf; T Graf
Journal:  Cell       Date:  1982-04       Impact factor: 41.582

7.  Pathology of colorectal adenomas: a colonoscopic survey.

Authors:  F Konishi; B C Morson
Journal:  J Clin Pathol       Date:  1982-08       Impact factor: 3.411

8.  Cloning and screening of sequences expressed in a mouse colon tumor.

Authors:  L H Augenlicht; D Kobrin
Journal:  Cancer Res       Date:  1982-03       Impact factor: 12.701

9.  An improved procedure for utilizing terminal transferase to add homopolymers to the 3' termini of DNA.

Authors:  G Deng; R Wu
Journal:  Nucleic Acids Res       Date:  1981-08-25       Impact factor: 16.971

10.  Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.

Authors:  J M Chirgwin; A E Przybyla; R J MacDonald; W J Rutter
Journal:  Biochemistry       Date:  1979-11-27       Impact factor: 3.162
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  3 in total

Review 1.  Molecular genetics of colorectal carcinoma.

Authors:  I B Kerr
Journal:  BMJ       Date:  1989-09-09

2.  Ribosomal protein genes are overexpressed in colorectal cancer: isolation of a cDNA clone encoding the human S3 ribosomal protein.

Authors:  K Pogue-Geile; J R Geiser; M Shu; C Miller; I G Wool; A I Meisler; J M Pipas
Journal:  Mol Cell Biol       Date:  1991-08       Impact factor: 4.272

3.  Isolation and preliminary characterisation of cDNA clones representing mRNAs associated with tumour progression and metastasis in colorectal cancer.

Authors:  P Elvin; I B Kerr; C S McArdle; G D Birnie
Journal:  Br J Cancer       Date:  1988-01       Impact factor: 7.640
  3 in total

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