Literature DB >> 3800905

Oxidative decarboxylation of 4-methylthio-2-oxobutyrate by branched-chain 2-oxo acid dehydrogenase complex.

S M Jones, S J Yeaman.   

Abstract

Highly purified branched-chain 2-oxo acid dehydrogenase complex (BCOADC) oxidizes 4-methylthio-2-oxobutyrate and 2-oxobutyrate, with Km values of 67 microM and 18 microM respectively. The Vmax. for oxidation of these substrates is 27% and 53% respectively of that for 3-methyl-2-oxobutyrate. Highly purified pyruvate dehydrogenase complex (PDC) oxidizes 2-oxobutyrate (Km 100 microM; Vmax. 49% of that for pyruvate) but not 4-methylthio-2-oxobutyrate, whereas 2-oxoglutarate dehydrogenase complex will not utilize either 2-oxo acid as substrate. BCOADC kinase is inhibited by both 4-methylthio-2-oxobutyrate and 2-oxobutyrate, with half-maximal inhibition by 45 microM and 50 microM respectively. Phosphorylation of BCOADC in isolated adipocytes is inhibited by both 4-methylthio-2-oxobutyrate and 2-oxobutyrate, consistent with their inhibitory action of BCOADC kinase. Phosphorylation of PDC is decreased by 2-oxobutyrate, but not by 4-methylthio-2-oxobutyrate.

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Year:  1986        PMID： 3800905      PMCID： PMC1147032          DOI： 10.1042/bj2370621

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  10 in total

1.  cAMP-dependent protein kinase and lipolysis in rat adipocytes. I. Cell preparation, manipulation, and predictability in behavior.

Authors:  R C Honnor; G S Dhillon; C Londos
Journal:  J Biol Chem       Date:  1985-12-05       Impact factor: 5.157

2.  Partial purification and properties of branched-chain 2-oxo acid dehydrogenase of ox liver.

Authors:  P J Parker; P J Randle
Journal:  Biochem J       Date:  1978-06-01       Impact factor: 3.857

3.  Role of branched-chain 2-oxo acid dehydrogenase and pyruvate dehydrogenase in 2-oxobutyrate metabolism.

Authors:  R Paxton; P W Scislowski; E J Davis; R A Harris
Journal:  Biochem J       Date:  1986-03-01       Impact factor: 3.857

4.  Purification and characterization of branched chain alpha-keto acid dehydrogenase complex of bovine kidney.

Authors:  F H Pettit; S J Yeaman; L J Reed
Journal:  Proc Natl Acad Sci U S A       Date:  1978-10       Impact factor: 11.205

5.  Purification of 2-oxo acid dehydrogenase multienzyme complexes from ox heart by a new method.

Authors:  C J Stanley; R N Perham
Journal:  Biochem J       Date:  1980-10-01       Impact factor: 3.857

6.  Purification and properties of pyruvate dehydrogenase kinase from bovine kidney.

Authors:  L R Stepp; F H Pettit; S J Yeaman; L J Reed
Journal:  J Biol Chem       Date:  1983-08-10       Impact factor: 5.157

7.  Rapid purification of bovine kidney branched-chain 2-oxoacid dehydrogenase complex containing endogenous kinase activity.

Authors:  R Lawson; K G Cook; S J Yeaman
Journal:  FEBS Lett       Date:  1983-06-27       Impact factor: 4.124

8.  Regulation of the branched chain 2-oxoacid dehydrogenase kinase reaction.

Authors:  K S Lau; H R Fatania; P J Randle
Journal:  FEBS Lett       Date:  1982-07-19       Impact factor: 4.124

9.  Phosphorylation of branched-chain 2-oxo acid dehydrogenase complex in isolated adipocytes. Effects of 2-oxo acids.

Authors:  S M Jones; S J Yeaman
Journal:  Biochem J       Date:  1986-05-15       Impact factor: 3.857

10.  Regulation of branched-chain alpha-ketoacid dehydrogenase kinase.

Authors:  R Paxton; R A Harris
Journal:  Arch Biochem Biophys       Date:  1984-05-15       Impact factor: 4.013
  10 in total
  17 in total

Review 1.  The 2-oxo acid dehydrogenase complexes: recent advances.

Authors:  S J Yeaman
Journal:  Biochem J       Date:  1989-02-01       Impact factor: 3.857

Review 2.  The biochemical and toxicological significance of hypermethionemia: new insights and clinical relevance.

Authors:  Joseph T Dever; Adnan A Elfarra
Journal:  Expert Opin Drug Metab Toxicol       Date:  2010-09-28       Impact factor: 4.481

3.  Impaired growth and neurological abnormalities in branched-chain alpha-keto acid dehydrogenase kinase-deficient mice.

Authors:  Mandar A Joshi; Nam Ho Jeoung; Mariko Obayashi; Eyas M Hattab; Eric G Brocken; Edward A Liechty; Michael J Kubek; Krishna M Vattem; Ronald C Wek; Robert A Harris
Journal:  Biochem J       Date:  2006-11-15       Impact factor: 3.857

4.  Methionine transamination in vivo.

Authors:  A J Cooper
Journal:  Biochem J       Date:  1989-09-01       Impact factor: 3.857

Review 5.  Maple syrup urine disease 1954 to 1993.

Authors:  F Peinemann; D J Danner
Journal:  J Inherit Metab Dis       Date:  1994       Impact factor: 4.982

6.  Methionine transamination--metabolic function and subcellular compartmentation.

Authors:  P W Scislowski; K Pickard
Journal:  Mol Cell Biochem       Date:  1993-12-08       Impact factor: 3.396

7.  Methionine metabolism by rat muscle and other tissues. Occurrence of a new carnitine intermediate.

Authors:  P W Scislowski; B M Hokland; W I Davis-van Thienen; J Bremer; E J Davis
Journal:  Biochem J       Date:  1987-10-01       Impact factor: 3.857

8.  L-methionine-dl-sulfoxide metabolism and toxicity in freshly isolated mouse hepatocytes: gender differences and inhibition with aminooxyacetic acid.

Authors:  Joseph T Dever; Adnan A Elfarra
Journal:  Drug Metab Dispos       Date:  2008-08-07       Impact factor: 3.922

9.  Lipoic acid-dependent oxidative catabolism of alpha-keto acids in mitochondria provides evidence for branched-chain amino acid catabolism in Arabidopsis.

Authors:  Nicolas L Taylor; Joshua L Heazlewood; David A Day; A Harvey Millar
Journal:  Plant Physiol       Date:  2004-02-05       Impact factor: 8.340

10.  Alternative methionine degradation via the transamination pathway: an option for therapy for homocystinuria due to cystathionine synthase deficiency.

Authors:  H J Blom; G H Boers; A Tangerman; W A Gahl; J M Trijbels
Journal:  J Inherit Metab Dis       Date:  1991       Impact factor: 4.982
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