Literature DB >> 3797244

Human lecithin-cholesterol acyltransferase gene: complete gene sequence and sites of expression.

J McLean, K Wion, D Drayna, C Fielding, R Lawn.   

Abstract

The human lecithin-cholesterol acyltransferase (LCAT) gene has been sequenced to completion. The gene is divided into six exons spanning approximately 4,200 bp. Exon five codes for amino acids homologous to the interfacial active site of several lipases, and also codes for an amphipathic alpha-helix resembling the carboxy terminus of apolipoprotein E. Blot hybridization data suggest that there is only one LCAT gene in humans. The 1550 base LCAT mRNA can be detected in liver and HepG2 (hepatocyte) cells, but not in small intestine, spleen, pancreas, placenta or adrenal tissue.

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Year:  1986        PMID： 3797244      PMCID： PMC311966          DOI： 10.1093/nar/14.23.9397

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  14 in total

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3.  Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.

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Journal:  Nucleic Acids Res       Date:  1984-09-25       Impact factor: 16.971

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Journal:  Annu Rev Biochem       Date:  1981       Impact factor: 23.643

5.  Human apolipoprotein E mRNA. cDNA cloning and nucleotide sequencing of a new variant.

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Journal:  J Biol Chem       Date:  1984-05-25       Impact factor: 5.157

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Authors:  C J Fielding; P E Fielding
Journal:  Proc Natl Acad Sci U S A       Date:  1981-06       Impact factor: 11.205

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Journal:  Proc Natl Acad Sci U S A       Date:  1986-04       Impact factor: 11.205

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Authors:  C C Shoulders; A R Kornblihtt; B S Munro; F E Baralle
Journal:  Nucleic Acids Res       Date:  1983-05-11       Impact factor: 16.971
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  26 in total

1.  Quantitative trait loci influencing cholesterol and phospholipid phenotypes map to chromosomes that contain genes regulating blood pressure in the spontaneously hypertensive rat.

Authors:  A Bottger; H A van Lith; V Kren; D Krenová; V Bílá; J Vorlícek; V Zídek; A Musilová; M Zdobinská; J M Wang; B F van Zutphen; T W Kurtz; M Pravenec
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2.  Recombinant human lecithin-cholesterol acyltransferase Fc fusion: analysis of N- and O-linked glycans and identification and elimination of a xylose-based O-linked tetrasaccharide core in the linker region.

Authors:  Chris Spahr; Justin J Kim; Sihong Deng; Paul Kodama; Zhen Xia; Jay Tang; Richard Zhang; Sophia Siu; Noi Nuanmanee; Bram Estes; Jennitte Stevens; Mingyue Zhou; Hsieng S Lu
Journal:  Protein Sci       Date:  2013-12       Impact factor: 6.725

3.  A molecular defect causing fish eye disease: an amino acid exchange in lecithin-cholesterol acyltransferase (LCAT) leads to the selective loss of alpha-LCAT activity.

Authors:  H Funke; A von Eckardstein; P H Pritchard; J J Albers; J J Kastelein; C Droste; G Assmann
Journal:  Proc Natl Acad Sci U S A       Date:  1991-06-01       Impact factor: 11.205

4.  Gene conversion as a secondary mechanism of short interspersed element (SINE) evolution.

Authors:  D H Kass; M A Batzer; P L Deininger
Journal:  Mol Cell Biol       Date:  1995-01       Impact factor: 4.272

5.  A new subfamily of recently retroposed human Alu repeats.

Authors:  J Jurka
Journal:  Nucleic Acids Res       Date:  1993-05-11       Impact factor: 16.971

6.  Mutagenically separated PCR (MS-PCR): a highly specific one step procedure for easy mutation detection.

Authors:  S Rust; H Funke; G Assmann
Journal:  Nucleic Acids Res       Date:  1993-08-11       Impact factor: 16.971

7.  Effects of site-directed mutagenesis at residues cysteine-31 and cysteine-184 on lecithin-cholesterol acyltransferase activity.

Authors:  O L Francone; C J Fielding
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-01       Impact factor: 11.205

8.  Fish eye syndrome: a molecular defect in the lecithin-cholesterol acyltransferase (LCAT) gene associated with normal alpha-LCAT-specific activity. Implications for classification and prognosis.

Authors:  H G Klein; S Santamarina-Fojo; N Duverger; M Clerc; M F Dumon; J J Albers; S Marcovina; H B Brewer
Journal:  J Clin Invest       Date:  1993-07       Impact factor: 14.808

9.  Genetic and phenotypic heterogeneity in familial lecithin: cholesterol acyltransferase (LCAT) deficiency. Six newly identified defective alleles further contribute to the structural heterogeneity in this disease.

Authors:  H Funke; A von Eckardstein; P H Pritchard; A E Hornby; H Wiebusch; C Motti; M R Hayden; C Dachet; B Jacotot; U Gerdes
Journal:  J Clin Invest       Date:  1993-02       Impact factor: 14.808

10.  Secretion of active human lecithin-cholesterol acyltransferase by insect cells infected with a recombinant baculovirus.

Authors:  D Chawla; J S Owen
Journal:  Biochem J       Date:  1995-07-01       Impact factor: 3.857
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