| Literature DB >> 378937 |
Abstract
A rapid method has been developed for isolation of ultraviolet-sensitive mutants of Escherichia coli, by inducing delay in the growth and/or division of repair-deficienct cells with low fluences of far-ultraviolet radiation, and killing with penicillin the repair-proficient cells, which continue to grow and divide. With this technique, we have achieved about a 3,000-fold enrichment for photoreactivation less (phr) cells and have isolated and characterized three phr mutants.Entities:
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Year: 1979 PMID: 378937 PMCID: PMC218104 DOI: 10.1128/jb.138.3.779-782.1979
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490