Literature DB >> 3788978

High molecular weight DNA from fixed cytogenetic preparations.

P E Barker, J R Testa, N Z Parsa, R Snyder.   

Abstract

Cell pellets that have been stored in routine clinical cytogenetic fixative were studied for the presence of intact DNA. A method for the isolation of high molecular weight DNA from fixed cytogenetic preparations of human leukocytes, bone marrow, and cell hybrid cultures is presented. DNA preparations from fixed pellets were cleaved with restriction enzymes, transferred to nitrocellulose filters after agarose gel electrophoresis, and hybridized to radiolabeled probes to demonstrate that fixed cell pellets could yield DNA of sufficient quality for Southern blot hybridization analysis. This protocol may be useful for molecular analysis of DNA from fixed cell pellets of patients who are unavailable for additional sampling.

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Year:  1986        PMID： 3788978      PMCID： PMC1684056     

Source DB:  PubMed          Journal:  Am J Hum Genet        ISSN： 0002-9297            Impact factor:   11.025


  14 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  A general method for isolation of high molecular weight DNA from eukaryotes.

Authors:  N Blin; D W Stafford
Journal:  Nucleic Acids Res       Date:  1976-09       Impact factor: 16.971

3.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

4.  Isolation and characterization of DNA from fixed cells and tissues.

Authors:  F E Arrighi; J Bergendahl; M Mandel
Journal:  Exp Cell Res       Date:  1968-04       Impact factor: 3.905

5.  Molecular cloning of the c-fms locus and its assignment to human chromosome 5.

Authors:  M F Roussel; C J Sherr; P E Barker; F H Ruddle
Journal:  J Virol       Date:  1983-12       Impact factor: 5.103

6.  Polymorphism of DNA sequence adjacent to human beta-globin structural gene: relationship to sickle mutation.

Authors:  Y W Kan; A M Dozy
Journal:  Proc Natl Acad Sci U S A       Date:  1978-11       Impact factor: 11.205

7.  Pre-B cell leukemia associated with chromosome translocation 1;19.

Authors:  A J Carroll; W M Crist; R T Parmley; M Roper; M D Cooper; W H Finley
Journal:  Blood       Date:  1984-03       Impact factor: 22.113

Review 8.  Construction of a genetic linkage map in man using restriction fragment length polymorphisms.

Authors:  D Botstein; R L White; M Skolnick; R W Davis
Journal:  Am J Hum Genet       Date:  1980-05       Impact factor: 11.025

9.  The formation apurinic acid from the desoxyribonucleic acid of calf thymus.

Authors:  C TAMM; M E HODES; E CHARGAFF
Journal:  J Biol Chem       Date:  1952-03       Impact factor: 5.486

10.  The human DNA locus related to the oncogene myb of avian myeloblastosis virus (AMV): molecular cloning and structural characterization.

Authors:  D Leprince; S Saule; C de Taisne; A Gegonne; A Begue; M Righi; D Stehelin
Journal:  EMBO J       Date:  1983       Impact factor: 11.598
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  5 in total

1.  Molecular genetic analysis of DNA obtained from fixed, air dried or paraffin embedded sources.

Authors:  K Grünewald; J Lyons; T E Hansen-Hagge; J W Janssen; H Feichtinger; C R Bartram
Journal:  Ann Hematol       Date:  1991-04       Impact factor: 3.673

2.  PCR amplification of specific DNA sequences from routinely fixed chromosomal spreads.

Authors:  P Jonveaux
Journal:  Nucleic Acids Res       Date:  1991-04-25       Impact factor: 16.971

3.  Efficient reidentification of chromosome slides from human metaphase spreads via microsatellite polymerase chain reaction.

Authors:  C Epplen; J T Epplen
Journal:  Chromosome Res       Date:  1995-03       Impact factor: 5.239

4.  Diagnosis of DiGeorge syndrome in nuclei released from archival autoptic heart specimens using fluorescence in situ hybridization.

Authors:  G Calabrese; R Mingarelli; P Francalanci; R Boldrini; G Palka; C Bosman; G Novelli; B Dallapiccola
Journal:  Hum Genet       Date:  1996-04       Impact factor: 4.132

5.  CGH and SNP array using DNA extracted from fixed cytogenetic preparations and long-term refrigerated bone marrow specimens.

Authors:  Ruth N Mackinnon; Carly Selan; Adrian Zordan; Meaghan Wall; Harshal Nandurkar; Lynda J Campbell
Journal:  Mol Cytogenet       Date:  2012-02-02       Impact factor: 2.009
  5 in total

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