Literature DB >> 378853

Enzyme-linked immunosorbent assay for immunoglobulin G and immunoglobulin A antibodies to Shigella flexneri antigens.

D F Keren.   

Abstract

An enzyme-linked immunosorbent assay has been developed to detect class-specific antibodies to Shigella flexneri lipopolysaccharide antigens. This enzyme-linked immunosorbent assay system has been used to measure antibodies present in serum or intestinal secretions without further purification. It is considerably more sensitive than passive hemagglutination, allowing detection of as little as 1.3 ng of specific immunoglobulin G antibody per ml in immune sera. Optimal conditions for this assay are outlined in this report.

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Year:  1979        PMID： 378853      PMCID： PMC414321          DOI： 10.1128/iai.24.2.441-448.1979

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  17 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  The role of Peyer's patches in the local immune response of rabbit ileum to live bacteria.

Authors:  D F Keren; P S Holt; H H Collins; P Gemski; S B Formal
Journal:  J Immunol       Date:  1978-06       Impact factor: 5.422

3.  The enzyme-linked immunosorbent assay (ELISA): a measure of antibody concentration or affinity.

Authors:  J E Butler; T L Feldbush; P L McGivern; N Stewart
Journal:  Immunochemistry       Date:  1978-02

4.  Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.

Authors:  E Engvall; P Perlmann
Journal:  J Immunol       Date:  1972-07       Impact factor: 5.422

5.  The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents.

Authors:  S Avrameas; T Ternynck
Journal:  Immunochemistry       Date:  1969-01

6.  Local (immunoglobulin A) immune response by the intestine to cholera toxin and its partial suppression with combined systemic and intra-intestinal immunization.

Authors:  J H Yardley; D F Keren; S R Hamilton; G D Brown
Journal:  Infect Immun       Date:  1978-02       Impact factor: 3.441

7.  Local antibody production in experimental pyelonephritis: amount, avidity, and immunoglobulin class.

Authors:  J Smith; J Holmgren; S Ahlstedt; L A Hanson
Journal:  Infect Immun       Date:  1974-09       Impact factor: 3.441

8.  Analysis of parameters affecting the solid phase radioimmunoassay quantitation of antibody to meningococcal antigens.

Authors:  W D Zollinger; J M Dalrymple; M S Artenstein
Journal:  J Immunol       Date:  1976-11       Impact factor: 5.422

9.  Enzyme-linked immunosorbent assay (ELISA) for titration of antibodies against Brucella abortus and Yersinia enterocolitica.

Authors:  H E Carlsson; B Hurvell; A A Lindberg
Journal:  Acta Pathol Microbiol Scand C       Date:  1976-06

10.  Determination of the optimal ammonium sulfate concentration for the fractionation of rabbit, sheep, horse, and goat antisera.

Authors:  G A Hebert; P L Pelham; B Pittman
Journal:  Appl Microbiol       Date:  1973-01
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  22 in total

1.  Immunoglobulin M, A, and G antibody response to lipopolysaccharide O antigen in symptomatic and asymptomatic Shigella infections.

Authors:  D Cohen; C Block; M S Green; G Lowell; I Ofek
Journal:  J Clin Microbiol       Date:  1989-01       Impact factor: 5.948

2.  Widal-type serology using live antigen for diagnosis of Shigella flexneri dysentery.

Authors:  H A Verbrugh; D R Mekkes; R P Verkooyen; J E Landheer
Journal:  Eur J Clin Microbiol       Date:  1986-10       Impact factor: 3.267

3.  Detection of antirotavirus immunoglobulins A, G, and M in swine colostrum, milk, and feces by enzyme-linked immunosorbent assay.

Authors:  G Corthier; J Franz
Journal:  Infect Immun       Date:  1981-02       Impact factor: 3.441

4.  Comparison of ELISA and RIA for measurement of pneumococcal antibodies before and after vaccination with 14-valent pneumococcal capsular polysaccharide vaccine.

Authors:  M Koskela; M Leinonen
Journal:  J Clin Pathol       Date:  1981-01       Impact factor: 3.411

5.  Immunoglobulin G and M antibodies to pneumococcal polysaccharides detected by enzyme-linked immunosorbent assay.

Authors:  D J Barrett; A J Ammann; S Stenmark; D W Wara
Journal:  Infect Immun       Date:  1980-02       Impact factor: 3.441

6.  No impairment of local intestinal immune response to keyhole limpet haemocyanin in the absence of Peyer's patches.

Authors:  S R Hamilton; D F Keren; J H Yardley; G Brown
Journal:  Immunology       Date:  1981-03       Impact factor: 7.397

7.  Intestinal immunoglobulin A responses in rabbits to a Salmonella typhi strain harboring a Shigella sonnei plasmid.

Authors:  D F Keren; H H Collins; L S Baron; D J Kopecko; S B Formal
Journal:  Infect Immun       Date:  1982-07       Impact factor: 3.441

8.  Enzyme-linked immunosorbent assay for detection of antibody to Treponema hyodysenteriae antigens.

Authors:  L A Joens; N A Nord; J M Kinyon; I T Egan
Journal:  J Clin Microbiol       Date:  1982-02       Impact factor: 5.948

9.  Secretory immunoglobulin A response following peroral priming and challenge with Shigella flexneri lacking the 140-megadalton virulence plasmid.

Authors:  D F Keren; R A McDonald; S B Formal
Journal:  Infect Immun       Date:  1986-12       Impact factor: 3.441

10.  Combined parenteral and oral immunization results in an enhanced mucosal immunoglobulin A response to Shigella flexneri.

Authors:  D F Keren; R A McDonald; J L Carey
Journal:  Infect Immun       Date:  1988-04       Impact factor: 3.441
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