Literature DB >> 3777432

Effect of zwitterionic buffers on measurement of small masses of protein with bicinchoninic acid.

V Kaushal, L D Barnes.   

Abstract

Linear standard curves were obtained for 1 to 10 micrograms of bovine serum albumin measured in the presence of 50 mM Mes, Mops, Pipes, Caps, Bes, Hepes, Heppso, and Tes zwitterionic buffers using bicinchoninic acid for detection. The zwitterionic buffers Ada, Tricine, and Bicine that strongly bind Cu2+. Tris, and Bistris, each at 50 mM, inhibited color development to prevent quantitative measurement of protein mass.

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Year:  1986        PMID： 3777432     DOI： 10.1016/0003-2697(86)90629-9

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  11 in total

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3.  Activation of intestinal brush border guanylate cyclase by aromatic disulphide compounds.

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4.  Dietary modulation of fatty acid profiles and oxidative status of rat hepatocyte nodules: effect of different n-6/n-3 fatty acid ratios.

Authors:  S Abel; M De Kock; C M Smuts; C de Villiers; S Swanevelder; W C A Gelderblom
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5.  Presence of functional receptors for the Escherichia coli heat-stable enterotoxin in the gastrointestinal tract of the chicken.

Authors:  L C Katwa; A A White
Journal:  Infect Immun       Date:  1992-09       Impact factor: 3.441

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7.  Altered lipid parameters in hepatic subcellular membrane fractions induced by fumonisin B1.

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8.  Naphthoquinone-dependent generation of superoxide radicals by quinone reductase isolated from the plasma membrane of soybean.

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9.  Membrane saturated fatty acids and disease progression in multiple sclerosis patients.

Authors:  G M Hon; M S Hassan; S J van Rensburg; S Abel; R T Erasmus; T Matsha
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10.  Assay of plant proteins with bicinchoninic acid for high resolution two-dimensional polyacrylamide gel electrophoresis.

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