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Literature DB >> 377228

Role of ribothymidine in the thermal stability of transfer RNA as monitored by proton magnetic resonance.

P Davanloo, M Sprinzl, K Watanabe, M Albani, H Kersten.

Abstract

In order to elucidate the functional role of the modified uridines at position 54 of tRNA, the 270 MHz high-field proton NMR spectra of methionine tRNAs from E. coli, from a mutant thereof, and from T. thermophilus, containing ribothymidine, uridine and 2-thioribothymidine, respectively, have been measured as a function of temperature. A comparison of the NMR melting profiles of the minor nucleosides from these tRNAs shows that the melting temperature of uridine containing tRNA is 6 degrees C lower than that of the wild type tRNA whereas that of the 2-thioribothymidine tRNA is 7 degrees C higher than that of the wild type tRNA. These results, therefore, demonstrate that these modifications serve for stabilization of the tertiary structure of tRNA.

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Year: 1979 PMID： 377228 PMCID： PMC327791 DOI： 10.1093/nar/6.4.1571

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

21 in total

1. Heat-induced stability of tRNA from an extreme thermophile, Thermus thermophilus.

Authors: K Watanabe; M Shinma; T Oshima; S Nishimura
Journal: Biochem Biophys Res Commun Date: 1976-10-04 Impact factor: 3.575

2. Demonstration of a tertiary interaction in solution between the extra arm and the D-stem in two different transfer RNA's by NMR.

Authors: P J Salemink; T Yamane; C W Hilbers
Journal: Nucleic Acids Res Date: 1977-11 Impact factor: 16.971

3. A nuclear magnetic resonance study of secondary and tertiary structure in yeast tRNAPhe.

Authors: G T Robillard; C E Tarr; F Vosman; B R Reid
Journal: Biochemistry Date: 1977-11-29 Impact factor: 3.162

4. 1H nuclear magnetic resonance studies of transfer RNA: the methyl and methylene resonances of baker's yeast phenylalanine transfer RNA and its fragments.

Authors: L S Kan; P O Ts'o; M Sprinzl; F vd Harr; F Cramer
Journal: Biochemistry Date: 1977-07-12 Impact factor: 3.162

5. The isolation and sequence analysis of transfer RNA: the use of plaskon chromatography (RPC-5).

Authors: B Roe; K Marcu; B Dudock
Journal: Biochim Biophys Acta Date: 1973-08-10

6. The molecular mechanism of thermal unfolding of Escherichia coli formylmethionine transfer RNA.

Authors: D M Crothers; P E Cole; C W Hilbers; R G Shulman
Journal: J Mol Biol Date: 1974-07-25 Impact factor: 5.469

7. Molecular structure of poly-2-thiouridylic acid, a double helix with non-equivalent polynucleotide chains.

Authors: S K Mazumdar; W Saenger
Journal: J Mol Biol Date: 1974-05-15 Impact factor: 5.469

8. Collection of published tRNA sequences.

Authors: M Sprinzl; F Grüter; D H Gauss
Journal: Nucleic Acids Res Date: 1978-05 Impact factor: 16.971

9. CD spectra of 5-methyl-2-thiouridine in tRNA-Met-f from an extreme thermophile.

Authors: K Watanabe; T Oshima; S Nishimura
Journal: Nucleic Acids Res Date: 1976-07 Impact factor: 16.971

10. Role of ribothymidine in mammalian tRNAPhe.

Authors: B A Roe; H Y Tsen
Journal: Proc Natl Acad Sci U S A Date: 1977-09 Impact factor: 11.205

37 in total

1. The nucleotide sequence of phenylalanine tRNA2 of Drosophila melanogaster: four isoacceptors with one basic sequence.

Authors: M Altwegg; E Kubli
Journal: Nucleic Acids Res Date: 1979-09-11 Impact factor: 16.971

Review 2. tRNA biology charges to the front.

Authors: Eric M Phizicky; Anita K Hopper
Journal: Genes Dev Date: 2010-09-01 Impact factor: 11.361

3. Identification of the enzyme responsible for N1-methylation of pseudouridine 54 in archaeal tRNAs.

Authors: Jan Philip Wurm; Marco Griese; Ute Bahr; Martin Held; Alexander Heckel; Michael Karas; Jörg Soppa; Jens Wöhnert
Journal: RNA Date: 2012-01-24 Impact factor: 4.942

4. The crystal structure of a novel SAM-dependent methyltransferase PH1915 from Pyrococcus horikoshii.

Authors: Warren Sun; Xiaohui Xu; Marina Pavlova; Aled M Edwards; Andrzej Joachimiak; Alexei Savchenko; Dinesh Christendat
Journal: Protein Sci Date: 2005-10-31 Impact factor: 6.725

5. Distinct Modified Nucleosides in tRNA^Trp from the Hyperthermophilic Archaeon Thermococcus kodakarensis and Requirement of tRNA m²G10/m² ₂G10 Methyltransferase (Archaeal Trm11) for Survival at High Temperatures.

Authors: Akira Hirata; Takeo Suzuki; Tomoko Nagano; Daishiro Fujii; Mizuki Okamoto; Manaka Sora; Todd M Lowe; Tamotsu Kanai; Haruyuki Atomi; Tsutomu Suzuki; Hiroyuki Hori
Journal: J Bacteriol Date: 2019-10-04 Impact factor: 3.490