Literature DB >> 3766942

Rapid preparation of bacteriophage DNA for sequence analysis in sets of 96 clones, using filtration.

I C Eperon.   

Abstract

A method is described for the preparation of single-stranded DNA from clones in bacteriophage M13 vectors. This procedure allows multiples of 96 clones to be processed at once, utilizing filtration to remove host cells and simplifying the treatment of bacteriophage pellets. The DNA produced can be used for sequencing of mutagenesis.

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Year:  1986        PMID： 3766942     DOI： 10.1016/0003-2697(86)90273-3

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  7 in total

1.  Alternative splicing of a human alpha-tropomyosin muscle-specific exon: identification of determining sequences.

Authors:  I R Graham; M Hamshere; I C Eperon
Journal:  Mol Cell Biol       Date:  1992-09       Impact factor: 4.272

2.  Enzymatic multiplex DNA sequencing.

Authors:  M Chee
Journal:  Nucleic Acids Res       Date:  1991-06-25       Impact factor: 16.971

3.  Single stranded rescue from phagemids in microtitre plates.

Authors:  W D Rawlinson; M S Chee; V Smith; B G Barrell
Journal:  Nucleic Acids Res       Date:  1991-09-11       Impact factor: 16.971

4.  High-throughput plasmid mini preparations facilitated by micro-mixing.

Authors:  W L Ng; M Schummer; F D Cirisano; R L Baldwin; B Y Karlan; L Hood
Journal:  Nucleic Acids Res       Date:  1996-12-15       Impact factor: 16.971

5.  The dependence of splicing efficiency on the length of 3' exon.

Authors:  A D Turnbull-Ross; A J Else; I C Eperon
Journal:  Nucleic Acids Res       Date:  1988-01-25       Impact factor: 16.971

6.  High-throughput detergent extraction of M13 subclones for fluorescent DNA sequencing.

Authors:  E R Mardis
Journal:  Nucleic Acids Res       Date:  1994-06-11       Impact factor: 16.971

7.  The muscle specific domain of mouse N-CAM: structure and alternative splicing patterns.

Authors:  M Hamshere; G Dickson; I Eperon
Journal:  Nucleic Acids Res       Date:  1991-09-11       Impact factor: 16.971
  7 in total

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