Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Transfer of the E. coli O6-methylguanine methyltransferase gene into repair-deficient human cells and restoration of cellular resistance to N-methyl-N'-nitro-N-nitrosoguanidine.

Literature DB >> 3762560

Transfer of the E. coli O6-methylguanine methyltransferase gene into repair-deficient human cells and restoration of cellular resistance to N-methyl-N'-nitro-N-nitrosoguanidine.

K Ishizaki, T Tsujimura, H Yawata, C Fujio, Y Nakabeppu, M Sekiguchi, M Ikenaga.

Abstract

We have constructed a plasmid on which the E. coli O6-methylguanine-DNA methyltransferase (MT) gene (ada gene) was linked with an SV40 promoter sequence and a poly(A) site. After transferring this plasmid into Mer- HeLa MR cells by DNA transfection, effective expression of E. coli MT was observed. Isolated stable transformant clones showed higher resistance to N-methyl-N'-nitro-N-nitrosoguanidine in colony formation and sister-chromatid exchange induction than HeLa MR cells.

Entities: Chemical Species

Mesh：

Substances：

Year: 1986 PMID： 3762560 DOI： 10.1016/0167-8817(86)90011-8

Source DB: PubMed Journal: Mutat Res ISSN： 0027-5107 Impact factor: 2.433

Keyword Cloud
Cited

11 in total

1. Relationship between O6-alkylguanine-DNA alkyltransferase activity and N-methyl-N'-nitro-N-nitrosoguanidine-induced mutation, transformation, and cytotoxicity in C3H/10T1/2 cells expressing exogenous alkyltransferase genes.

Authors: E von Hofe; L Fairbairn; G P Margison
Journal: Proc Natl Acad Sci U S A Date: 1992-12-01 Impact factor: 11.205

2. Competency in mismatch repair prohibits clonal expansion of cancer cells treated with N-methyl-N'-nitro-N-nitrosoguanidine.

Authors: J M Carethers; M T Hawn; D P Chauhan; M C Luce; G Marra; M Koi; C R Boland
Journal: J Clin Invest Date: 1996-07-01 Impact factor: 14.808

Review 3. Identification of human genes involved in repair and tolerance of DNA damage.

Authors: B Kaina; G Fritz; T Coquerelle
Journal: Radiat Environ Biophys Date: 1991 Impact factor: 1.925

4. The GT to GC single nucleotide polymorphism at the beginning of an alternative exon 2C of human MTH1 gene confers an amino terminal extension that functions as a mitochondrial targeting signal.

Authors: Yasunari Sakai; Hisanobu Oda; Daisuke Yoshimura; Masato Furuichi; Dongchon Kang; Shigenori Iwai; Toshiro Hara; Yusaku Nakabeppu
Journal: J Mol Med (Berl) Date: 2006-04-11 Impact factor: 4.599

5. Depletion of mammalian O6-alkylguanine-DNA alkyltransferase activity by O6-benzylguanine provides a means to evaluate the role of this protein in protection against carcinogenic and therapeutic alkylating agents.

Authors: M E Dolan; R C Moschel; A E Pegg
Journal: Proc Natl Acad Sci U S A Date: 1990-07 Impact factor: 11.205

10. Complementation of sensitivity to alkylating agents in Escherichia coli and Chinese hamster ovary cells by expression of a cloned bacterial DNA repair gene.

Authors: H Kataoka; J Hall; P Karran
Journal: EMBO J Date: 1986-12-01 Impact factor: 11.598

Transfer of the E. coli O6-methylguanine methyltransferase gene into repair-deficient human cells and restoration of cellular resistance to N-methyl-N'-nitro-N-nitrosoguanidine.

1. Relationship between O6-alkylguanine-DNA alkyltransferase activity and N-methyl-N'-nitro-N-nitrosoguanidine-induced mutation, transformation, and cytotoxicity in C3H/10T1/2 cells expressing exogenous alkyltransferase genes.

2. Competency in mismatch repair prohibits clonal expansion of cancer cells treated with N-methyl-N'-nitro-N-nitrosoguanidine.

Review 3. Identification of human genes involved in repair and tolerance of DNA damage.

4. The GT to GC single nucleotide polymorphism at the beginning of an alternative exon 2C of human MTH1 gene confers an amino terminal extension that functions as a mitochondrial targeting signal.

5. Depletion of mammalian O6-alkylguanine-DNA alkyltransferase activity by O6-benzylguanine provides a means to evaluate the role of this protein in protection against carcinogenic and therapeutic alkylating agents.

6. The Escherichia coli AlkB protein protects human cells against alkylation-induced toxicity.

7. Overexpressed human metallothionein IIA gene protects Chinese hamster ovary cells from killing by alkylating agents.

8. Analysis of nuclear transport signals in the human apurinic/apyrimidinic endonuclease (APE1/Ref1).

Review 9. The 1987 Walter Hubert lecture. Regulation and deficiencies in DNA repair.

10. Complementation of sensitivity to alkylating agents in Escherichia coli and Chinese hamster ovary cells by expression of a cloned bacterial DNA repair gene.