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Literature DB >> 375227

Nucleotide sequence of the region of an origin of replication of the antibiotic resistance plasmid R6K.

Abstract

A 2.1-kilobase segment of the antibiotic resistance plasmid R6K carries sufficient information to replicate as a plasmid in Escherichia coli. This segment contains a functional origin of replication and a structural gene for a protein, designated pi, that is required for the initiation of R6K replication. The nucleotide sequence of a 520-base-pair portion of this 2.1-kilobase segment that includes the functional origin of replication and the region adjacent to the start of the pi structural gene was determined. A striking feature of the sequence is the presence of seven 22-base-pair direct repeats joined in tandem in the region adjacent to the start of the pi gene. A possible role of the tandem repeats in the regulation of expression of the pi protein and the control of initiation of replication of the plasmid R6K is discussed.

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Year: 1979 PMID： 375227 PMCID： PMC383207 DOI： 10.1073/pnas.76.3.1150

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

38 in total

1. The genome of simian virus 40.

Authors: V B Reddy; B Thimmappaya; R Dhar; K N Subramanian; B S Zain; J Pan; P K Ghosh; M L Celma; S M Weissman
Journal: Science Date: 1978-05-05 Impact factor: 47.728

2. Similarity of nucleotide sequences around the origin of DNA replication in mouse polyoma virus and simian virus 40.

Authors: E Soeda; G Kimura; K Miura
Journal: Proc Natl Acad Sci U S A Date: 1978-01 Impact factor: 11.205

3. Nucleotide sequence of the DNA replication origin for human papovavirus BKV: sequence and structural homology with SV40.

Authors: R Dhar; C J Lai; G Khoury
Journal: Cell Date: 1978-02 Impact factor: 41.582

4. Complete nucleotide sequence of SV40 DNA.

Authors: W Fiers; R Contreras; G Haegemann; R Rogiers; A Van de Voorde; H Van Heuverswyn; J Van Herreweghe; G Volckaert; M Ysebaert
Journal: Nature Date: 1978-05-11 Impact factor: 49.962

5. Construction of plasmid R6K derivatives in vitro: characterization of the R6K replication region.

Authors: R Kolter; D R Helinski
Journal: Plasmid Date: 1978-09 Impact factor: 3.466

6. The nucleotide sequence surrounding the origin of DNA replication of Col E1.

Authors: D Bastia
Journal: Nucleic Acids Res Date: 1977-09 Impact factor: 16.971

7. Nucleotide sequence of the origin of replication of the Escherichia coli K-12 chromosome.

Authors: M Meijer; E Beck; F G Hansen; H E Bergmans; W Messer; K von Meyenburg; H Schaller
Journal: Proc Natl Acad Sci U S A Date: 1979-02 Impact factor: 11.205

8. Nucleotide sequence of Escherichia coli K-12 replication origin.

Authors: K Sugimoto; A Oka; H Sugisaki; M Takanami; A Nishimura; Y Yasuda; Y Hirota
Journal: Proc Natl Acad Sci U S A Date: 1979-02 Impact factor: 11.205

9. Requirement of a plasmid-encoded protein for replication in vitro of plasmid R6K.

Authors: M Inuzuka; D R Helinski
Journal: Proc Natl Acad Sci U S A Date: 1978-11 Impact factor: 11.205

10. Identification of simian virus 40 protein A.

Authors: K Rundell; J K Collins; P Tegtmeyer; H L Ozer; C J Lai; D Nathans
Journal: J Virol Date: 1977-02 Impact factor: 5.103

49 in total

1. Dimers of pi protein bind the A+T-rich region of the R6K gamma origin near the leading-strand synthesis start sites: regulatory implications.

Authors: R Krüger; M Filutowicz
Journal: J Bacteriol Date: 2000-05 Impact factor: 3.490

2. pi protein- and ATP-dependent transitions from 'closed' to 'open' complexes at the gamma ori of plasmid R6K.

Authors: Ricardo Krüger; Marcin Filutowicz
Journal: Nucleic Acids Res Date: 2003-10-15 Impact factor: 16.971

3. Identification of a novel promoter in the replication control region of plasmid R6K.

Authors: P Mukerji; A Greener; M Filutowicz
Journal: J Bacteriol Date: 1992-07 Impact factor: 3.490

4. Initiator protein pi can bind independently to two domains of the gamma origin core of plasmid R6K: the direct repeats and the A+T-rich segment.

Authors: I Levchenko; M Filutowicz
Journal: Nucleic Acids Res Date: 1996-05-15 Impact factor: 16.971

8. Altered (copy-up) forms of initiator protein pi suppress the point mutations inactivating the gamma origin of plasmid R6K.

Authors: M Urh; Y Flashner; A Shafferman; M Filutowicz
Journal: J Bacteriol Date: 1995-12 Impact factor: 3.490

9. Nucleotide sequence and functional properties of DNA encoding incompatibility in the broad host-range plasmid R1162.

Authors: L S Lin; R J Meyer
Journal: Mol Gen Genet Date: 1984

10. Primary structure of the essential replicon of the plasmid pSC101.

Authors: C Vocke; D Bastia
Journal: Proc Natl Acad Sci U S A Date: 1983-11 Impact factor: 11.205