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Literature DB >> 370832

Nucleotide sequence of Escherichia coli K-12 replication origin.

K Sugimoto, A Oka, H Sugisaki, M Takanami, A Nishimura, Y Yasuda, Y Hirota.

Abstract

From subfragments of an EcoRI fragment (9 kilobase pairs) that contained the replication origin of the Escherichia coli chromosome and had been cloned as a recombinant with a nonreplicating DNA fragment coding for ampicillin resistance, small derivative plasmids were constructed. The smallest of these, pTSO151, contained a segment of 463 base pairs as the chromosomal component. Another plasmid, pSY134, constructed from BamHI digests of the EcoRI fragment and mini-F(pMF21), contained a region of 422 base pairs identical with a corresponding region in pTSO151. We conclude that the replication origin of E. coli chromosome is located within this 422-base-pair segment. The nucleotide sequence of this segment is presented.

Entities: Chemical Gene Species

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Year: 1979 PMID： 370832 PMCID： PMC382991 DOI： 10.1073/pnas.76.2.575

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

29 in total

9. Transcription in vivo within the replication origin of the Escherichia coli chromosome: a mechanism for activating initiation of replication.

Authors: T Asai; C P Chen; T Nagata; M Takanami; M Imai
Journal: Mol Gen Genet Date: 1992-01

10. Initiation of chromosomal DNA replication which is stimulated without oversupply of DnaA protein in Escherichia coli.

Authors: T Katayama; T Nagata
Journal: Mol Gen Genet Date: 1991-05

Nucleotide sequence of Escherichia coli K-12 replication origin.

1. DNA regions essential for the function of a bacteriophage fd promoter.

2. Synthetic ColE1 plasmids carrying genes for cell division in Escherichia coli.

3. Fine cleavage map of a small colicin E1 plasmid carrying genes responsible for replication and colicin E1 immunity.

4. Origin of replication of colicin E1 plasmid DNA.

5. Restriction endonuclease mapping and mutagenesis of the F sex factor replication region.

6. Nucleotide sequence of the origin of replication of the Escherichia coli K-12 chromosome.

7. Origin of replication, oriC, or the Escherichia coli chromosome on specialized transducing phages lambda asn.

8. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

9. Cloning and mapping of the replication origin of Escherichia coli.

10. Nucleotide sequences of the separate origins of synthesis of bacteriophage G4 viral and complementary DNA strands.

1. Deoxyribonucleic acid methylation and chromatin organization in Tetrahymena thermophila.

2. The hemimethylated replication origin of Escherichia coli can be initiated in vitro.

3. Cosmid-derived map of E. coli strain BHB2600 in comparison to the map of strain W3110.

4. Mutations in the DnaA binding sites of the replication origin of Escherichia coli.

5. The 55-kilodalton protein in an oriC complex fraction is glycogen synthase.

6. Replication origin mutations affecting binding of pSC101 plasmid-encoded Rep initiator protein.

7. Characterization of Helicobacter pylori dapE and construction of a conditionally lethal dapE mutant.

8. An easier method for locating replication origins based on real-time polymerase chain reaction.

9. Transcription in vivo within the replication origin of the Escherichia coli chromosome: a mechanism for activating initiation of replication.

10. Initiation of chromosomal DNA replication which is stimulated without oversupply of DnaA protein in Escherichia coli.