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Literature DB >> 374344

Mapping of ilvO loci of Escherichia coli K-12 with bacteriophage lambda dilv.

Abstract

A set of lambda dilv phage have been used in a deletion mapping procedure to determine the location of two previously characterized ilvO alleles. In contrast to earlier conclusions derived from three-factor crosses and episome-shortening techniques with phage P1, the order found is ilvG-ilvO-ilvEDA. A three-factor cross with phage P1 is described that is not consistent with this location for an ilvO allele. Further analysis of this particular three-factor cross revealed than an artifact attributable to a mutual syntrophism had skewed the apparent frequency of inheritance of the ilvO locus. The role of mutual syntrophism is discussed as a source of mapping errors for the ilvO locus. The value of this set of lambda dilv phage and this mapping procedure for obtaining comparatively unambiguous data on the locations of the ilv structural and regulatory genes is demonstrated.

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Year: 1979 PMID： 374344 PMCID： PMC218306 DOI： 10.1128/jb.137.3.1234-1242.1979

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

12 in total

7. Expression of a valine-resistant acetolactate synthase activity mediated by the ilv O and ilv G genes of Escherichia coli K-12.

Authors: R Favre; A Wiater; S Puppo; M Iaccarino
Journal: Mol Gen Genet Date: 1976-02-02

8. Isoleucine and valine metabolism in Escherichia coli. XXII. A pleiotropic mutation affecting induction of isomeroreductase activity.

Authors: W J Pledger; H E Umbarger
Journal: J Bacteriol Date: 1973-04 Impact factor: 3.490

Mapping of ilvO loci of Escherichia coli K-12 with bacteriophage lambda dilv.

1. The regulation of the ilv ADGE operon: evidence for positive control by threonine deaminase.

2. Suppressors of a genetic regulatory mutation affecting isoleucine-valine biosynthesis in Escherichia coli K-12.

3. Regulation of isoleucine-valine biosynthesis in an ilvDAC deletion strain of Escherichia coli K-12.

4. Regulation of transaminase C synthesis in Escherichia coli: conditional leucine auxotrophy.

5. Polarity and the regulation of the ilv gene cluster in Escherichia coli strain K-12.

6. Physical organization of the ilvEDAC genes of Escherichia coli strain K-12.

7. Expression of a valine-resistant acetolactate synthase activity mediated by the ilv O and ilv G genes of Escherichia coli K-12.

8. Isoleucine and valine metabolism in Escherichia coli. XXII. A pleiotropic mutation affecting induction of isomeroreductase activity.

9. REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. I. GENETIC DEREPRESSION OF ENZYME FORMATION.

10. REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. II. IDENTIFICATION OF TWO OPERATOR GENES.

1. Physical characterization of ilv-lac fusions.

2. Absence of significant membrane localization of the proteins coded by the ilvGEDAC genes of Escherichia coli K-12.

3. Identification of a protein of 15,000 daltons related to isoleucine-valine biosynthesis in Escherichia coli K-12.

4. Physical and genetic localization of ilv regulatory sites in lambda ilv bacteriophages.

5. Genetical and structural analysis of a group of lambda ilv and lambda rho transducing phages.

6. Identification of the protein products of the rrnC, ilv, rho region of the Escherichia coli K-12 chromosome.

Review 7. Linkage map of Escherichia coli K-12, edition 6.

8. Cloning of the ilvA538 gene coding for feedback-hypersensitive threonine deaminase from Escherichia coli K-12.

9. Genetic organization of the Salmonella typhimurium ilv gene cluster.