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Literature DB >> 159287

Physical characterization of ilv-lac fusions.

Abstract

Electron microscopic heteroduplex analysis and comparative restriction digests have been used to characterize lambda p123(209), the complementary pair of phages used in the Casadaban technique of gene fusion. Derivatives of lambda 1(209) constructed to carry fusions of the lac genes to the control regions of the ilvC and ilvEDA operons were also analyzed. These physical maps have provided confirmation of the genetic models for these constructions and physical specifications important in interpreting the behavior of these ilv-lac fusions.

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Year: 1979 PMID： 159287 PMCID： PMC216803 DOI： 10.1128/jb.140.1.251-260.1979

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

10 in total

1. Analysis of bacteriophage mu and lambda-mu hybrid DNAs by specific endonucleases.

Authors: B Allet; A I Bukhari
Journal: J Mol Biol Date: 1975-03-15 Impact factor: 5.469

2. New Map Location of ilvO in ESCHERICHIA COLI.

Authors: B M Cohen; E W Jones
Journal: Genetics Date: 1976-06 Impact factor: 4.562

3. Mapping of ilvO loci of Escherichia coli K-12 with bacteriophage lambda dilv.

Authors: D W Patin; D H Calhoun
Journal: J Bacteriol Date: 1979-03 Impact factor: 3.490

4. Restriction endonuclease mapping of the Escherichia coli K12 chromosome in the vicinity of the ilv genes.

Authors: G J Childs; H Ohtsubo; E Ohtsubo; F Sonnenberg; M Freundlich
Journal: J Mol Biol Date: 1977-11-25 Impact factor: 5.469

5. The phi80 lambdadilv phage and its use to detect messenger RNA related to isoleucine-valine metabolism.

Authors: F L Schiavo; R Favre; T Kasai; A Cascino; J Guardiola; L Caro; M Iaccarino
Journal: J Mol Biol Date: 1975-12-15 Impact factor: 5.469

6. Polarity and the regulation of the ilv gene cluster in Escherichia coli strain K-12.

Authors: J M Smith; D E Smolin; H E Umbarger
Journal: Mol Gen Genet Date: 1976-10-18

7. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu.

Authors: M J Casadaban
Journal: J Mol Biol Date: 1976-07-05 Impact factor: 5.469

8. Nucleotide sequences at the ends of bacteriophage Mu DNA.

Authors: B Allet
Journal: Nature Date: 1978-08-10 Impact factor: 49.962

9. Physical organization of the ilvEDAC genes of Escherichia coli strain K-12.

Authors: G M McCorkle; T D Leathers; H E Umbarger
Journal: Proc Natl Acad Sci U S A Date: 1978-01 Impact factor: 11.205

10. Isolation of transducing bacteriophages for the histidine and isoleucine-valine operons in Escherichia coli K-12.

Authors: A Avitabile; S Carlomagno-Cerillo; R Favvre; F Blasi
Journal: J Bacteriol Date: 1972-10 Impact factor: 3.490

10 in total

15 in total

1. Specificity of attenuation control in the ilvGMEDA operon of Escherichia coli K-12.

Authors: J W Chen; D C Bennett; H E Umbarger
Journal: J Bacteriol Date: 1991-04 Impact factor: 3.490

Review 2. Uses of lac fusions for the study of biological problems.

Authors: T J Silhavy; J R Beckwith
Journal: Microbiol Rev Date: 1985-12

3. Isolation and analysis of two Escherichia coli K-12 ilv attenuator deletion mutants with high-level constitutive expression of an ilv-lac fusion operon.

Authors: D C Bennett; H E Umbarger
Journal: J Bacteriol Date: 1984-03 Impact factor: 3.490

4. Isolation of an aroF-lac plasmid by recombination in vivo.

Authors: C S Cobbett; J Pittard
Journal: J Bacteriol Date: 1984-01 Impact factor: 3.490

5. Physical and genetic localization of ilv regulatory sites in lambda ilv bacteriophages.

Authors: J E Gray; D C Bennett; H E Umbarger; D H Calhoun
Journal: J Bacteriol Date: 1982-03 Impact factor: 3.490

6. Restriction map of the Escherichia coli malA region and identification of the malT product.

Authors: O Raibaud; M Schwartz
Journal: J Bacteriol Date: 1980-08 Impact factor: 3.490

7. Molecular cloning of an osmoregulatory locus in Escherichia coli: increased proU gene dosage results in enhanced osmotolerance.

Authors: J Gowrishankar; P Jayashree; K Rajkumari
Journal: J Bacteriol Date: 1986-12 Impact factor: 3.490

8. Lambda placMu: a transposable derivative of bacteriophage lambda for creating lacZ protein fusions in a single step.

Authors: E Bremer; T J Silhavy; J M Weisemann; G M Weinstock
Journal: J Bacteriol Date: 1984-06 Impact factor: 3.490

9. Regulation of ilvEDA expression occurs upstream of ilvG in Escherichia coli: additional evidence for an ilvGEDA operon.

Authors: C S Subrahmanyam; J D Noti; H E Umbarger
Journal: J Bacteriol Date: 1980-10 Impact factor: 3.490

10. Construction from Mu d1 (lac Apr) lysogens of lambda bacteriophage bearing promoter-lac fusions: isolation of lambda ppheA-lac.

Authors: J Gowrishankar; J Pittard
Journal: J Bacteriol Date: 1982-06 Impact factor: 3.490