Literature DB >> 3737750

A digitonin-based procedure for the isolation of mitochondrial DNA from mammalian cells.

N Howell, M S Nalty, J Appel.   

Abstract

A procedure for the isolation of closed circular mitochondrial DNA (mtDNA) from cells permeabilized with digitonin is described. Compared to the standard procedure in which cells are broken after osmotic swelling, the digitonin-based procedure is more consistent and results in higher mtDNA yields.

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Year:  1986        PMID： 3737750     DOI： 10.1016/0147-619x(86)90083-1

Source DB:  PubMed          Journal:  Plasmid        ISSN： 0147-619X            Impact factor:   3.466


  3 in total

1.  Evaluation of individual particle capillary electrophoresis experiments via quantile analysis.

Authors:  Christofer E Whiting; Edgar A Arriaga
Journal:  J Chromatogr A       Date:  2007-05-05       Impact factor: 4.759

2.  Determining under- and oversampling of individual particle distributions in microfluidic electrophoresis with orthogonal laser-induced fluorescence detection.

Authors:  Christofer E Whiting; Rajat A Dua; Ciarán F Duffy; Edgar A Arriaga
Journal:  Electrophoresis       Date:  2008-04       Impact factor: 3.535

3.  Subcellular partitioning of MRP RNA assessed by ultrastructural and biochemical analysis.

Authors:  K Li; C S Smagula; W J Parsons; J A Richardson; M Gonzalez; H K Hagler; R S Williams
Journal:  J Cell Biol       Date:  1994-03       Impact factor: 10.539
  3 in total

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