| Literature DB >> 3737417 |
R F Pasternack, P Garrity, B Ehrlich, C B Davis, E J Gibbs, G Orloff, A Giartosio, C Turano.
Abstract
The ionic strength dependences of the binding of tetrakis (4-N-methylpyridyl)porphine (H2TMpyP) to poly(dG-dC) and calf thymus DNA have been determined. For the former system the results are typical of other intercalators, i.e., a plot of log K vs log [Na+] is linear albeit with a slope which suggests that the "effective charge" of the porphyrin is closer to two than the formal charge of +4. For calf thymus DNA, the binding profile is not completely compatible with the predictions of condensation theory. Whereas the avidity of binding does decrease with increasing [Na+] as predicted, of greater interest is the relocation of the porphyrin from GC-rich regions to AT-rich regions as the ionic strength increases.Entities:
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Year: 1986 PMID： 3737417 PMCID： PMC311600 DOI： 10.1093/nar/14.14.5919
Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971