Literature DB >> 3728962

Amino acid analysis by HPLC: optimized conditions for chromatography of phenylthiocarbamyl derivatives.

R F Ebert.   

Abstract

A method for analysis of phenylthiocarbamyl-amino acids by HPLC is described. Solvent A contains 0.05 M sodium acetate plus triethylamine (2.75 ml/liter) adjusted to pH 6.40 with phosphoric acid. Solvent B consists of 50% solvent A, 40% acetonitrile, and 10% methanol. The stationary phase is a 3-micron Spherisorb ODS-2 column (4.6 X 100 mm). A new sample can be injected every 27 min. Variation of the triethylamine concentration from 1 to 4 ml/liter is shown to affect column selectivity in a predictable manner, and this provides a rational basis for optimizing chromatographic conditions. Resolution of certain hydrophilic amino acids (Arg, Thr, Ala, and Pro), and mobile phase stability are improved over previously described procedures.

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Year:  1986        PMID： 3728962     DOI： 10.1016/0003-2697(86)90010-2

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  17 in total

1.  A novel lysine 2,3-aminomutase encoded by the yodO gene of bacillus subtilis: characterization and the observation of organic radical intermediates.

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2.  Microheterogeneous Cytosolic High-Mobility Group Proteins from Broccoli Co-Purify with and Are Phosphorylated by Casein Kinase II.

Authors:  L. J. Klimczak; A. R. Cashmore
Journal:  Plant Physiol       Date:  1994-07       Impact factor: 8.340

3.  Basis for the equilibrium constant in the interconversion of l-lysine and l-beta-lysine by lysine 2,3-aminomutase.

Authors:  Dawei Chen; Justinn Tanem; Perry A Frey
Journal:  Biochim Biophys Acta       Date:  2006-12-20

4.  28 kDa adenosine-binding proteins of brain and other tissues.

Authors:  K Ravid; R A Rosenthal; S R Doctrow; J M Lowenstein
Journal:  Biochem J       Date:  1989-03-15       Impact factor: 3.857

5.  Neuropeptide-degrading endopeptidase activity of locust (Schistocerca gregaria) synaptic membranes.

Authors:  R E Isaac
Journal:  Biochem J       Date:  1988-11-01       Impact factor: 3.857

6.  Proline accumulation in maize (Zea mays L.) primary roots at low water potentials. II. Metabolic source of increased proline deposition in the elongation zone

Authors: 
Journal:  Plant Physiol       Date:  1999-04       Impact factor: 8.340

7.  Isolation and identification of a diuretic hormone from the tobacco hornworm, Manduca sexta.

Authors:  H Kataoka; R G Troetschler; J P Li; S J Kramer; R L Carney; D A Schooley
Journal:  Proc Natl Acad Sci U S A       Date:  1989-04       Impact factor: 11.205

8.  Measurement of apolipoprotein B in various cell lines: correlation between intracellular levels and rates of secretion.

Authors:  A Bakillah; Z Zhou; J Luchoomun; M M Hussain
Journal:  Lipids       Date:  1997-10       Impact factor: 1.880

9.  The cellular eye lens and crystallins of cubomedusan jellyfish.

Authors:  J Piatigorsky; J Horwitz; T Kuwabara; C E Cutress
Journal:  J Comp Physiol A       Date:  1989-02       Impact factor: 1.836

10.  Argininosuccinate synthetase is a functional target for a snake venom anti-hypertensive peptide: role in arginine and nitric oxide production.

Authors:  Juliano R Guerreiro; Claudiana Lameu; Eduardo F Oliveira; Clécio F Klitzke; Robson L Melo; Edlaine Linares; Ohara Augusto; Jay W Fox; Ivo Lebrun; Solange M T Serrano; Antonio C M Camargo
Journal:  J Biol Chem       Date:  2009-06-02       Impact factor: 5.157
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