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Literature DB >> 372560

Involvement of DNA gyrase in replication and transcription of bacteriophage T7 DNA.

Abstract

Growth of bacteriophage T7 is inhibited by the antibiotic coumermycin A1, an inhibitor of the Escherichia coli DNA gyrase. Since growth of the phage is insensitive to the antibiotic in strains containing a coumermycin-resistant DNA gyrase, this enzyme appears to be required for phage growth. We have investigated the effect of coumermycin on the kinetics of DNA, RNA, and protein synthesis during T7 infection. DNA synthesis is completely inhibited by the antibiotic. In addition, coumermycin significantly inhibits transcription of late but not early genes. Thus, E. coli DNA gyrase may play an important role in transcription as well as in replication of T7 DNA.

Entities: Chemical Species

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Year: 1979 PMID： 372560 PMCID： PMC353186

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

26 in total

1. Mechanism of action of nalidixic acid: purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme.

Authors: A Sugino; C L Peebles; K N Kreuzer; N R Cozzarelli
Journal: Proc Natl Acad Sci U S A Date: 1977-11 Impact factor: 11.205

1. Defective transcription of the right end of bacteriophage T7 DNA during an abortive infection of F plasmid-containing Escherichia coli.

Authors: P J Beck; I J Molineux
Journal: J Bacteriol Date: 1991-02 Impact factor: 3.490

2. Isolation and characterisation of a strain carrying a conditional lethal mutation in the cou gene of Escherichia coli K12.

Authors: E Orr; N F Fairweather; I B Holland; R H Pritchard
Journal: Mol Gen Genet Date: 1979

6. The gyrB gene product functions in both initiation and chain polymerization of Escherichia coli chromosome replication: suppression of the initiation deficiency in gyrB-ts mutants by a class of rpoB mutations.

Authors: M Filutowicz; P Jonczyk
Journal: Mol Gen Genet Date: 1983

Involvement of DNA gyrase in replication and transcription of bacteriophage T7 DNA.

1. Mechanism of action of nalidixic acid: purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme.

2. Involvement of DNA gyrase in bacteriophage T7 DNA replication.

3. Role of DNA gyrase in phiX replicative-form replication in vitro.

4. Involement of supertwisted DNA in integrative recombination of bacteriophage lambda.

5. Superhelical Escherichia coli DNA: relaxation by coumermycin.

6. Effect of circularity and superhelicity on transcription from bacteriophagelambda DNA.

7. Interactions between twisted DNAs and enzymes: the effects of superhelical turns.

8. Fast sedimenting bacteriophage T7 DNA from T7-infected Escherichia coli.

9. Folded, concatenated genomes as replication intermediates of bacteriophage T7 DNA.

10. Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase.

1. Defective transcription of the right end of bacteriophage T7 DNA during an abortive infection of F plasmid-containing Escherichia coli.

2. Isolation and characterisation of a strain carrying a conditional lethal mutation in the cou gene of Escherichia coli K12.

3. Involvement of DNA gyrase in bacteriophage T7 growth.

4. DNA supercoiling and suppression of the leu-500 promoter mutation.

Review 5. Biology of bacterial deoxyribonucleic acid topoisomerases.

6. The gyrB gene product functions in both initiation and chain polymerization of Escherichia coli chromosome replication: suppression of the initiation deficiency in gyrB-ts mutants by a class of rpoB mutations.

Review 7. Bacteriophage T3 and bacteriophage T7 virus-host cell interactions.

8. Bacteriophage T7 late promoters: construction and in vitro transcription properties of deletion mutants.

9. Multiplicity reactivation of bacteriophage T7 inactivated by methyl methanesulfonate.

10. Involvement of host DNA gyrase in growth of bacteriophage T5.