Literature DB >> 3718491

The chemical mechanism of sheep liver 6-phosphogluconate dehydrogenase. A Schiff-base intermediate is not involved.

C M Topham, K Dalziel.   

Abstract

[2-18O]Ribulose 5-phosphate was prepared and shown to be converted enzymically by 6-phosphogluconate dehydrogenase from sheep liver into 6-phosphogluconate with complete retention of the heavy isotope. This finding unequivocally excludes the possibility of a Schiff-base mechanism for the enzyme. The involvement of metal ions has already been excluded, and other possible mechanisms are discussed. The enzyme was purified by an improved large-scale procedure, which is briefly described.

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Year:  1986        PMID： 3718491      PMCID： PMC1146624          DOI： 10.1042/bj2340671

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  27 in total

1.  The purification and properties of NADP-dependent isocitrate dehydrogenase from ox-heart mitochondria.

Authors:  N Macfarlane; B Mathews; K Dalziel
Journal:  Eur J Biochem       Date:  1977-04-15

2.  THE STEREOCHEMISTRY OF DECARBOXYLATION OF ISOCITRATE BY ISOCITRIC ACID DEHYDROGENASE.

Authors:  G E LIENHARD; I A ROSE
Journal:  Biochemistry       Date:  1964-02       Impact factor: 3.162

3.  Enzymatic formation of xylulose 5-phosphate from ribose 5-phosphate in spleen.

Authors:  G ASHWELL; J HICKMAN
Journal:  J Biol Chem       Date:  1957-05       Impact factor: 5.157

4.  The effect of inorganic salts on the ketone decomposition of oxaloacetic acid.

Authors:  H A Krebs
Journal:  Biochem J       Date:  1942-04       Impact factor: 3.857

5.  Studies of 6-phosphogluconate dehydrogenase from sheep liver. 2. Kinetics of the oxidative-decarboxylation reaction, coenzyme binding and analyses for metals.

Authors:  R H Villet; K Dalziel
Journal:  Eur J Biochem       Date:  1972-05-23

Review 6.  The mechanism of action of aldolases.

Authors:  D E Morse; B L Horecker
Journal:  Adv Enzymol Relat Areas Mol Biol       Date:  1968

7.  Crystalline 6-phosphogluconate dehydrogenase from sheep liver.

Authors:  M Silverberg; K Dalziel
Journal:  Eur J Biochem       Date:  1973-10-05

8.  An examination of potential matrices for the affinity chromatography of NADP+-linked dehydrogenases with special reference to 6-phosphogluconate dehydrogenase.

Authors:  P Griffiths; A Houlton; M J Adams; M J Harvey; P D Dean
Journal:  Biochem J       Date:  1977-03-01       Impact factor: 3.857

9.  The nature of the carbon dioxide substrate and equilibrium constant of the 6-phosphogluconate dehydrogenase reaction.

Authors:  R H Villet; K Dalziel
Journal:  Biochem J       Date:  1969-12       Impact factor: 3.857

10.  The three dimensional structure of sheep liver 6-phosphogluconate dehydrogenase at 2.6 A resolution.

Authors:  M J Adams; I G Archibald; C E Bugg; A Carne; S Gover; J R Helliwell; R W Pickersgill; S W White
Journal:  EMBO J       Date:  1983       Impact factor: 11.598
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  3 in total

1.  A model of the pentose phosphate pathway in rat liver cells.

Authors:  L Sabate; R Franco; E I Canela; J J Centelles; M Cascante
Journal:  Mol Cell Biochem       Date:  1995-01-12       Impact factor: 3.396

2.  Kinetic properties of hexose-monophosphate dehydrogenases. II. Isolation and partial purification of 6-phosphogluconate dehydrogenase from rat liver and kidney cortex.

Authors:  F J Corpas; L García-Salguero; J B Barroso; F Aranda; J A Lupiáñez
Journal:  Mol Cell Biochem       Date:  1995-03-23       Impact factor: 3.396

Review 3.  6-Phosphogluconate dehydrogenase and its crystal structures.

Authors:  Stefania Hanau; John R Helliwell
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2022-02-23       Impact factor: 1.056
  3 in total

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