Literature DB >> 15840

The purification and properties of NADP-dependent isocitrate dehydrogenase from ox-heart mitochondria.

N Macfarlane, B Mathews, K Dalziel.   

Abstract

The purification of NADP-linked isocitrate dehydrogenase from ox heart mitochondria is described. The molecular weight from gel filtration, sedimentation equilibrium and gel electrophoresis is 90000+/-4000, and there are two subunits in the molecule each of which binds NADPH with enhancement of the coenzyme fluorescence. The amino-acid composition is reported, and the absorption coefficient, A1/280%, estimated from dry weight measurements is 11.8 cm-1.

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Year:  1977        PMID： 15840     DOI： 10.1111/j.1432-1033.1977.tb11424.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  8 in total

1.  Molecular weight of NADP-dependent isocitrate dehydrogenase from rat brain cytosol under normoxia and hypoxia.

Authors:  U Rafałowska; A Pastuszko
Journal:  Neurochem Res       Date:  1979-04       Impact factor: 3.996

2.  Transient kinetics of nicotinamide-adenine dinucleotide phosphate-linked isocitrate dehydrogenase from bovine heart mitochondria.

Authors:  K Dalziel; N McFerran; B Matthews; C H Reynolds
Journal:  Biochem J       Date:  1978-06-01       Impact factor: 3.857

3.  Equilibrium binding of coenzymes and substrates to nicotinamide-adenine dinucleotide phosphate-linked isocitrate dehydrogenase from bovine heart mitochondria.

Authors:  C H Reynolds; P W Kuchel; K Dalziel
Journal:  Biochem J       Date:  1978-06-01       Impact factor: 3.857

4.  The chemical mechanism of sheep liver 6-phosphogluconate dehydrogenase. A Schiff-base intermediate is not involved.

Authors:  C M Topham; K Dalziel
Journal:  Biochem J       Date:  1986-03-15       Impact factor: 3.857

5.  NAD-Linked Isocitrate Dehydrogenase: Isolation, Purification, and Characterization of the Protein from Pea Mitochondria.

Authors:  C A McIntosh; D J Oliver
Journal:  Plant Physiol       Date:  1992-09       Impact factor: 8.340

6.  Purification and structural properties of isozymes of isocitrate dehydrogenase from the mouse.

Authors:  B Pegoraro; J H Yuan; C Y Lee
Journal:  Mol Cell Biochem       Date:  1979-02-09       Impact factor: 3.396

7.  Novel NADP-linked isocitrate dehydrogenase present in peroxisomes of n-alkane-utilizing yeast, Candida tropicalis: comparison with mitochondrial NAD-linked isocitrate dehydrogenase.

Authors:  S Yamamoto; H Atomi; M Ueda; A Tanaka
Journal:  Arch Microbiol       Date:  1995-02       Impact factor: 2.552

8.  Some properties of pig kidney-cortex aldehyde reductase.

Authors:  F F Morpeth; F M Dickinson
Journal:  Biochem J       Date:  1980-11-01       Impact factor: 3.857
  8 in total

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