Literature DB >> 3713851

Fluorescence detection in automated DNA sequence analysis.

L M Smith, J Z Sanders, R J Kaiser, P Hughes, C Dodd, C R Connell, C Heiner, S B Kent, L E Hood.   

Abstract

We have developed a method for the partial automation of DNA sequence analysis. Fluorescence detection of the DNA fragments is accomplished by means of a fluorophore covalently attached to the oligonucleotide primer used in enzymatic DNA sequence analysis. A different coloured fluorophore is used for each of the reactions specific for the bases A, C, G and T. The reaction mixtures are combined and co-electrophoresed down a single polyacrylamide gel tube, the separated fluorescent bands of DNA are detected near the bottom of the tube, and the sequence information is acquired directly by computer.

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Year:  1986        PMID： 3713851     DOI： 10.1038/321674a0

Source DB:  PubMed          Journal:  Nature        ISSN： 0028-0836            Impact factor:   49.962


  244 in total

1.  A novel neuron-enriched homolog of the erythrocyte membrane cytoskeletal protein 4.1.

Authors:  L D Walensky; S Blackshaw; D Liao; C C Watkins; H U Weier; M Parra; R L Huganir; J G Conboy; N Mohandas; S H Snyder
Journal:  J Neurosci       Date:  1999-08-01       Impact factor: 6.167

2.  Measurement of locus copy number by hybridisation with amplifiable probes.

Authors:  J A Armour; C Sismani; P C Patsalis; G Cross
Journal:  Nucleic Acids Res       Date:  2000-01-15       Impact factor: 16.971

3.  Automated parallel DNA sequencing on multiple channel microchips.

Authors:  S Liu; H Ren; Q Gao; D J Roach; R T Loder; T M Armstrong; Q Mao; I Blaga; D L Barker; S B Jovanovich
Journal:  Proc Natl Acad Sci U S A       Date:  2000-05-09       Impact factor: 11.205

4.  Intrinsic fluorescence from DNA can be enhanced by metallic particles.

Authors:  J R Lakowicz; B Shen; Z Gryczynski; S D'Auria; I Gryczynski
Journal:  Biochem Biophys Res Commun       Date:  2001-09-07       Impact factor: 3.575

Review 5.  DNA probes using fluorescence resonance energy transfer (FRET): designs and applications.

Authors:  V V Didenko
Journal:  Biotechniques       Date:  2001-11       Impact factor: 1.993

6.  The interplay of biology and technology.

Authors:  S Fields
Journal:  Proc Natl Acad Sci U S A       Date:  2001-08-21       Impact factor: 11.205

7.  Biosynthesis of riboflavin: an unusual riboflavin synthase of Methanobacterium thermoautotrophicum.

Authors:  S Eberhardt; S Korn; F Lottspeich; A Bacher
Journal:  J Bacteriol       Date:  1997-05       Impact factor: 3.490

8.  Sequence information can be obtained from single DNA molecules.

Authors:  Ido Braslavsky; Benedict Hebert; Emil Kartalov; Stephen R Quake
Journal:  Proc Natl Acad Sci U S A       Date:  2003-03-21       Impact factor: 11.205

9.  Digital detection of genetic mutations using SPC-sequencing.

Authors:  Hameer Ruparel; Michael E Ulz; Sobin Kim; Jingyue Ju
Journal:  Genome Res       Date:  2004-02       Impact factor: 9.043

10.  Sequences and characterization of hupU and hupV genes of Bradyrhizobium japonicum encoding a possible nickel-sensing complex involved in hydrogenase expression.

Authors:  L K Black; C Fu; R J Maier
Journal:  J Bacteriol       Date:  1994-11       Impact factor: 3.490
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