Literature DB >> 3706727

A fluorometric assay of the degree of modification of protein primary amines with polyethylene glycol.

S J Stocks, A J Jones, C W Ramey, D E Brooks.   

Abstract

An alternative primary amine assay utilizing 4-phenylspiro[furan-2(3H),1'-phthalan]-3,3'-dione (fluorescamine) is compared with the frequently applied trinitrobenzenesulfonic acid assay as a means of estimating protein primary amine modification with cyanuric chloride attachment of polyethylene glycol (PEG). The results of the two assays are compared for three different proteins and several advantages of the fluorescamine assay are emphasized. In particular, the fluorescamine assay was found to be unaffected by free PEG up to 0.08% in the assay mixture. The fluorescamine assay can be used to measure the partition coefficients of native or PEG-modified protein in two-phase aqueous polymer systems.

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Year:  1986        PMID： 3706727     DOI： 10.1016/0003-2697(86)90520-8

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  15 in total

1.  Accurate evaluation method of the polymer content in monomethoxy(polyethylene glycol) modified proteins based on amino acid analysis.

Authors:  L Sartore; P Caliceti; O Schiavon; C Monfardini; F M Veronese
Journal:  Appl Biochem Biotechnol       Date:  1991-12       Impact factor: 2.926

2.  Glucose-binding property of pegylated concanavalin A.

Authors:  J J Kim; K Park
Journal:  Pharm Res       Date:  2001-06       Impact factor: 4.200

3.  Effect of glycosylation on the catalytic and conformational stability of homologous alpha-amylases.

Authors:  Soundararajan Srimathi; Gurunathan Jayaraman
Journal:  Protein J       Date:  2005-02       Impact factor: 2.371

4.  IgG antibody response to polyethylene glycol-modified adenosine deaminase in patients with adenosine deaminase deficiency.

Authors:  S Chaffee; A Mary; E R Stiehm; D Girault; A Fischer; M S Hershfield
Journal:  J Clin Invest       Date:  1992-05       Impact factor: 14.808

5.  Modification of PEGylated enzyme with glutaraldehyde can enhance stability while avoiding intermolecular crosslinking.

Authors:  D W Ritter; J M Newton; M J McShane
Journal:  RSC Adv       Date:  2014-06-17       Impact factor: 3.361

6.  Use of site-directed mutagenesis to enhance the epitope-shielding effect of covalent modification of proteins with polyethylene glycol.

Authors:  M S Hershfield; S Chaffee; L Koro-Johnson; A Mary; A A Smith; S A Short
Journal:  Proc Natl Acad Sci U S A       Date:  1991-08-15       Impact factor: 11.205

7.  Reversible modification of thiol-containing polypeptides with poly (ethylene glycol) through formation of mixed disulfide bonds. The case of papaya proteinase III.

Authors:  T Musu; M Azarkan; J Brygier; C Paul; J Vincentelli; D Baeyens-Volant; C Guermant; M Nijs; Y Looze
Journal:  Appl Biochem Biotechnol       Date:  1996-03       Impact factor: 2.926

8.  Preparation and preliminary characterization of poly(ethylene glycol)-pepstatin conjugate.

Authors:  J Brygier; J Vincentelli; M Nijs; C Guermant; C Paul; D Baeyens-Volant; Y Looze
Journal:  Appl Biochem Biotechnol       Date:  1994-04       Impact factor: 2.926

9.  Structure of poly(ethylene glycol)-modified horseradish peroxidase in organic solvents: infrared amide I spectral changes upon protein dehydration are largely caused by protein structural changes and not by water removal per se.

Authors:  Wasfi Al-Azzam; Emil A Pastrana; Yancy Ferrer; Qing Huang; Reinhard Schweitzer-Stenner; Kai Griebenow
Journal:  Biophys J       Date:  2002-12       Impact factor: 4.033

10.  Impact of large aggregated uricases and PEG diol on accelerated blood clearance of PEGylated canine uricase.

Authors:  Chun Zhang; Kai Fan; Xuefeng Ma; Dongzhi Wei
Journal:  PLoS One       Date:  2012-06-26       Impact factor: 3.240
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