Literature DB >> 3700631

Evaluation of a commercial kit for the routine detection of Clostridium difficile cytotoxin by tissue culture.

T C Wu, S M Gersch.   

Abstract

The Toxi-titer microtiter plate system (Bartels Immunodiagnostic Supplies, Inc., Bellevue, Wash.) is a simplified procedure for detecting the cytotoxin produced by Clostridium difficile in stool filtrates. In a parallel study of 74 stool specimens, results from the Toxin-titer system compared favorably with those from the conventional system. Our experience with the Toxin-titer system in testing 540 stool specimens was, in general, satisfactory, although a few problems with toxin control did occur. The method requires no specialized experience in tissue culture techniques and can therefore be adopted for routine use in clinical laboratories.

Entities:  

Mesh：

Substances：

Year:  1986        PMID： 3700631      PMCID： PMC362840          DOI： 10.1128/jcm.23.4.792-793.1986

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  2 in total

1.  Enzyme immunoassays for detection of Clostridium difficile toxins A and B in fecal specimens.

Authors:  B E Laughon; R P Viscidi; S L Gdovin; R H Yolken; J G Bartlett
Journal:  J Infect Dis       Date:  1984-05       Impact factor: 5.226

2.  Evaluation of the usefulness of counterimmunoelectrophoresis for diagnosis of Clostridium difficile-associated colitis in clinical specimens.

Authors:  T C Wu; J C Fung
Journal:  J Clin Microbiol       Date:  1983-04       Impact factor: 5.948
  2 in total
  10 in total

1.  Evaluation of an enzyme immunoassay for detection of Clostridium difficile toxin A.

Authors:  K Tsimidis; A E Simor
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1992-06       Impact factor: 3.267

Review 2.  Clostridium difficile: its disease and toxins.

Authors:  D M Lyerly; H C Krivan; T D Wilkins
Journal:  Clin Microbiol Rev       Date:  1988-01       Impact factor: 26.132

3.  Isolation of Clostridium difficile and detection of cytotoxin in the feces of diarrheic foals in the absence of antimicrobial treatment.

Authors:  R L Jones; W S Adney; R K Shideler
Journal:  J Clin Microbiol       Date:  1987-07       Impact factor: 5.948

4.  Pseudomembranous colitis: how useful is endoscopy?

Authors:  J M Bergstein; A Kramer; D H Wittman; C Aprahamian; E J Quebbeman
Journal:  Surg Endosc       Date:  1990       Impact factor: 4.584

Review 5.  Clostridium difficile: clinical disease and diagnosis.

Authors:  F C Knoop; M Owens; I C Crocker
Journal:  Clin Microbiol Rev       Date:  1993-07       Impact factor: 26.132

6.  Comparison of five cultural procedures for isolation of Clostridium difficile from stools.

Authors:  L M Marler; J A Siders; L C Wolters; Y Pettigrew; B L Skitt; S D Allen
Journal:  J Clin Microbiol       Date:  1992-02       Impact factor: 5.948

7.  Development of a rapid enzyme immunoassay for Clostridium difficile toxin A and its use in the diagnosis of C. difficile-associated disease.

Authors:  J R DiPersio; F J Varga; D L Conwell; J A Kraft; K J Kozak; D H Willis
Journal:  J Clin Microbiol       Date:  1991-12       Impact factor: 5.948

8.  Comparison of two commercially available enzyme immunoassays for detection of Clostridium difficile in stool specimens.

Authors:  S S Altaie; P Meyer; D Dryja
Journal:  J Clin Microbiol       Date:  1994-01       Impact factor: 5.948

9.  Concurrence of Clostridium difficile toxin A enzyme-linked immunosorbent assay, fecal lactoferrin assay, and clinical criteria with C. difficile cytotoxin titer in two patient cohorts.

Authors:  M A Schleupner; D C Garner; K M Sosnowski; C J Schleupner; L J Barrett; E Silva; D Hirsch; R L Guerrant
Journal:  J Clin Microbiol       Date:  1995-07       Impact factor: 5.948

10.  Laboratory diagnosis of Clostridium difficile-associated gastrointestinal disease: comparison of a monoclonal antibody enzyme immunoassay for toxins A and B with a monoclonal antibody enzyme immunoassay for toxin A only and two cytotoxicity assays.

Authors:  G V Doern; R T Coughlin; L Wu
Journal:  J Clin Microbiol       Date:  1992-08       Impact factor: 5.948
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.