Literature DB >> 6101243

Enzyme immunoassays for detection of Clostridium difficile toxins A and B in fecal specimens.

B E Laughon, R P Viscidi, S L Gdovin, R H Yolken, J G Bartlett.   

Abstract

Enzyme immunoassays for detection of Clostridium difficile toxins A and B were developed with use of a double-sandwich microtiter plate format. Each assay was specific for its respective toxin and was sensitive to 0.1 ng of toxin. Neither assay was reactive with 13 other species of clostridia. One hundred fifty fecal specimens submitted for tissue culture cytotoxicity assay were evaluated by enzyme-linked immunosorbent assay (ELISA). Of the 79 tissue culture-positive specimens, 72 (91%) were positive in the A assay, 63 (80%) were positive in the B assay, and 75 (95%) were positive in either assay. Specimens with tissue culture titers of greater than or equal to 10(3) were uniformly positive in both assays. The specificities of the toxin A and B ELISAs were 98.6% and 100%, respectively. An ELISA for both toxins could serve as a substitute for the tissue culture cytotoxicity assay.

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Year:  1984        PMID： 6101243     DOI： 10.1093/infdis/149.5.781

Source DB:  PubMed          Journal:  J Infect Dis        ISSN： 0022-1899            Impact factor:   5.226


  36 in total

1.  Monoclonal antibodies specific for Clostridium difficile toxin B and their use in immunoassays.

Authors:  F Müller; C Stiegler; U Hadding
Journal:  J Clin Microbiol       Date:  1992-06       Impact factor: 5.948

2.  Evaluation of a commercial enzyme immunoassay kit for the detection of Clostridium difficile toxin A.

Authors:  S P Borriello; T Vale; J S Brazier; S Hyde; E Chippeck
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1992-04       Impact factor: 3.267

3.  Evaluation of a new commercial Clostridium difficile toxin A enzyme immunoassay using diarrhoeal stools.

Authors:  M Delmée; T Mackey; A Hamitou
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1992-03       Impact factor: 3.267

Review 4.  Clostridial enteric diseases of domestic animals.

Authors:  J G Songer
Journal:  Clin Microbiol Rev       Date:  1996-04       Impact factor: 26.132

5.  Analysis of latex agglutination test for Clostridium difficile toxin A (D-1) and differentiation between C difficile toxins A and B and latex reactive protein.

Authors:  S P Borriello; F E Barclay; P J Reed; A R Welch; J D Brown; D W Burdon
Journal:  J Clin Pathol       Date:  1987-05       Impact factor: 3.411

6.  Characterization of toxins A and B of Clostridium difficile with monoclonal antibodies.

Authors:  D M Lyerly; C J Phelps; J Toth; T D Wilkins
Journal:  Infect Immun       Date:  1986-10       Impact factor: 3.441

7.  Multicenter evaluation of a new enzyme immunoassay for detection of Clostridium difficile enterotoxin A.

Authors:  P C De Girolami; P A Hanff; K Eichelberger; L Longhi; H Teresa; J Pratt; A Cheng; J M Letourneau; G M Thorne
Journal:  J Clin Microbiol       Date:  1992-05       Impact factor: 5.948

8.  Diarrheal disease and DRGs.

Authors:  Peter H Gilligan
Journal:  Clin Microbiol Newsl       Date:  2004-01-22

9.  Six rapid tests for direct detection of Clostridium difficile and its toxins in fecal samples compared with the fibroblast cytotoxicity assay.

Authors:  David K Turgeon; Thomas J Novicki; John Quick; LaDonna Carlson; Pat Miller; Bruce Ulness; Anne Cent; Rhoda Ashley; Ann Larson; Marie Coyle; Ajit P Limaye; Brad T Cookson; Thomas R Fritsche
Journal:  J Clin Microbiol       Date:  2003-02       Impact factor: 5.948

10.  Monospecific antibodies for detection of Clostridium difficile enterotoxin.

Authors:  P Rautenberg; F Stender; U Ullmann
Journal:  Eur J Clin Microbiol       Date:  1987-02       Impact factor: 3.267
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