Literature DB >> 3692927

Immunocytochemical localization of serine: pyruvate aminotransferase in peroxisomes of the human liver parenchymal cells.

S Yokota1, T Oda, A Ichiyama.   

Abstract

The localization of serine:pyruvate aminotransferase (SPT) in human liver was investigated by indirect immunoenzyme and protein A-gold techniques. By light microscopy, diaminobenzidine reaction product was present in cytoplasmic granules of the parenchymal cells. By electron microscopy, gold particles indicating the antigenic sites for SPT were exclusively confined to peroxisomes but not to mitochondria. By double labeling technique, both peroxisomal marker enzyme, catalase and SPT were detected in the same peroxisomes. Quantitative analysis of the labeling density showed that SPT is contained only in peroxisomes. The results indicate that in human liver most of SPT is contained in the peroxisomes.

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Year:  1987        PMID： 3692927     DOI： 10.1007/bf00492477

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  28 in total

1.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

2.  Cytochemical detection of catalase with 3,3'-diaminobenzidine. A quantitative reinvestigation of the optimal conditions.

Authors:  M LeHir; V Herzog; H D Fahimi
Journal:  Histochemistry       Date:  1979-11

3.  Light microscopic immunocytochemical demonstration of peroxisomal enzymes in epon sections.

Authors:  J A Litwin; S Yokota; T Hashimoto; H D Fahimi
Journal:  Histochemistry       Date:  1984

4.  Peroxisomal localization of serine:pyruvate aminotransferase in human liver.

Authors:  T Noguchi; Y Takada
Journal:  J Biol Chem       Date:  1978-11-10       Impact factor: 5.157

5.  The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.

Authors:  R C Graham; M J Karnovsky
Journal:  J Histochem Cytochem       Date:  1966-04       Impact factor: 2.479

6.  Zellweger syndrome: biochemical and morphological studies on two patients treated with clofibrate.

Authors:  P B Lazarow; V Black; H Shio; Y Fujiki; A K Hajra; N S Datta; B S Bangaru; J Dancis
Journal:  Pediatr Res       Date:  1985-12       Impact factor: 3.756

7.  Immunocytochemical demonstration of serine: pyruvate amino-transferase in peroxisomes and mitochondria of rat kidney.

Authors:  S Yokota; T Oda
Journal:  Histochemistry       Date:  1985

8.  [Morphology and diagnosis of Zellweger syndrome. A contribution to combined cytochemical-finestructural identification of peroxisomes in autopsy material and frozen liver tissue with case report].

Authors:  J Müller-Höcker; K Bise; W Endres; G Hübner
Journal:  Virchows Arch A Pathol Anat Histol       Date:  1981

9.  More useful maleimide compounds for the conjugation of Fab' to horseradish peroxidase through thiol groups in the hinge.

Authors:  S Hashida; M Imagawa; S Inoue; K H Ruan; E Ishikawa
Journal:  J Appl Biochem       Date:  1984 Feb-Apr

10.  Structure, composition, physical properties, and turnover of proliferated peroxisomes. A study of the trophic effects of Su-13437 on rat liver.

Authors:  F Leighton; L Coloma; C Koenig
Journal:  J Cell Biol       Date:  1975-11       Impact factor: 10.539
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  6 in total

Review 1.  Primary hyperoxaluria type 1: genotypic and phenotypic heterogeneity.

Authors:  C J Danpure; P R Jennings; P Fryer; P E Purdue; J Allsop
Journal:  J Inherit Metab Dis       Date:  1994       Impact factor: 4.982

2.  Enzymological and mutational analysis of a complex primary hyperoxaluria type 1 phenotype involving alanine:glyoxylate aminotransferase peroxisome-to-mitochondrion mistargeting and intraperoxisomal aggregation.

Authors:  C J Danpure; P E Purdue; P Fryer; S Griffiths; J Allsop; M J Lumb; K M Guttridge; P R Jennings; J I Scheinman; S M Mauer
Journal:  Am J Hum Genet       Date:  1993-08       Impact factor: 11.025

3.  Human peroxisomal L-alanine: glyoxylate aminotransferase. Evolutionary loss of a mitochondrial targeting signal by point mutation of the initiation codon.

Authors:  Y Takada; N Kaneko; H Esumi; P E Purdue; C J Danpure
Journal:  Biochem J       Date:  1990-06-01       Impact factor: 3.857

Review 4.  Studies on a unique organelle localization of a liver enzyme, serine:pyruvate (or alanine:glyoxylate) aminotransferase.

Authors:  Arata Ichiyama
Journal:  Proc Jpn Acad Ser B Phys Biol Sci       Date:  2011       Impact factor: 3.493

5.  An enzyme trafficking defect in two patients with primary hyperoxaluria type 1: peroxisomal alanine/glyoxylate aminotransferase rerouted to mitochondria.

Authors:  C J Danpure; P J Cooper; P J Wise; P R Jennings
Journal:  J Cell Biol       Date:  1989-04       Impact factor: 10.539

6.  Mammalian alanine/glyoxylate aminotransferase 1 is imported into peroxisomes via the PTS1 translocation pathway. Increased degeneracy and context specificity of the mammalian PTS1 motif and implications for the peroxisome-to-mitochondrion mistargeting of AGT in primary hyperoxaluria type 1.

Authors:  A Motley; M J Lumb; P B Oatey; P R Jennings; P A De Zoysa; R J Wanders; H F Tabak; C J Danpure
Journal:  J Cell Biol       Date:  1995-10       Impact factor: 10.539
  6 in total

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