Literature DB >> 3689350

Expression of the cDNA encoding for mouse sperm-specific lactate dehydrogenase C in Chinese-hamster ovary cells.

E W Hou1, I Sakai, S S Li.   

Abstract

The cloned cDNA encoding for mouse sperm-specific lactate dehydrogenase C (LDH-C) was inserted immediately downstream to the MMTV 5' LTR promoter, and it was shown to synthesize mouse LDH-C polypeptide in Chinese-hamster ovary cells. The mouse LDH-C subunit and the endogenous Chinese-hamster LDH-A subunit formed in vivo a heterotetrameric LDH-A3C1 isoenzyme, and this novel isoenzyme exhibited enzymic activity utilizing lactate as substrate.

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Year:  1987        PMID： 3689350      PMCID： PMC1148395          DOI： 10.1042/bj2470241

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  17 in total

1.  Non-cross-reactivity of antibodies to murine LDH-C4 with LDH-A4 and LDH-B4.

Authors:  Z G Liang; J A Shelton; E Goldberg
Journal:  J Exp Zool       Date:  1986-12

2.  Immunochemical homologies among vertebrate lactate-dehydrogenase isozymes.

Authors:  R S Holmes; R K Scopes
Journal:  Eur J Biochem       Date:  1974-03-15

3.  Infertility in female rabbits immunized with lactate dehydrogenase X.

Authors:  E Goldberg
Journal:  Science       Date:  1973-08-03       Impact factor: 47.728

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Immunochemical specificity of lactate dehydrogenase-X.

Authors:  E Goldberg
Journal:  Proc Natl Acad Sci U S A       Date:  1971-02       Impact factor: 11.205

6.  Detection of deletion mutations in pSV2gpt-transformed cells.

Authors:  K R Tindall; L F Stankowski; R Machanoff; A W Hsie
Journal:  Mol Cell Biol       Date:  1984-07       Impact factor: 4.272

7.  Molecular features and immunological properties of lactate dehydrogenase C4 isozymes from mouse and rat testes.

Authors:  S S Li; R J Feldmann; M Okabe; Y C Pan
Journal:  J Biol Chem       Date:  1983-06-10       Impact factor: 5.157

8.  Evolutionary relationships of vertebrate lactate dehydrogenase isozymes A4 (muscle), B4 (heart), and C4 (testis).

Authors:  S S Li; W M Fitch; Y C Pan; F S Sharief
Journal:  J Biol Chem       Date:  1983-06-10       Impact factor: 5.157

9.  Amino acid composition and properties of crystalline lactate dehydrogenase X from mouse testes.

Authors:  E Goldberg
Journal:  J Biol Chem       Date:  1972-04-10       Impact factor: 5.157

10.  Molecular cloning and nucleotide sequence of the cDNA for sperm-specific lactate dehydrogenase-C from mouse.

Authors:  I Sakai; F S Sharief; S S Li
Journal:  Biochem J       Date:  1987-03-01       Impact factor: 3.857
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