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Literature DB >> 368563

Method of isolation of cysteine constitutive mutants of the cysteine regulon in Salmonella typhimurium.

Abstract

A method for selection of constitutive cysB mutation is described which takes advantage of the resistance of cysteine constitutive mutants to 1,2,4-triazole. Since cysM cysK double mutants are cysteine auxotrophs, by selecting for triazole resistance in cysM strains, mutants arising under this condition also should be constitutive for cysteine biosynthesis. Genetic analysis of mutants isolated by this technique showed that their mutational sites are located in the cysB region. Biochemical assays of cysteine enzymes, sulphite reductase and O-acetylserine sulfhydrylase of the mutants showed the derepressed level of these enzymes and the lack or slight repression by 1-cysteine.

Entities: Chemical Species

Mesh：

Substances：
Triazoles
Cysteine

Year: 1978 PMID： 368563 DOI： 10.1007/bf00332529

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

19 in total

10. Positive control of sulphate reduction in Escherichia coli. Isolation, characterization and mapping oc cysteineless mutants of E. coli K12.

Authors: M C Jones-Mortimer
Journal: Biochem J Date: 1968-12 Impact factor: 3.857

5 in total

1. Analysis of merodiploids of the cysB region in Salmonella typhimurium.

Authors: G Jagura; D Hulanicka
Journal: Mol Gen Genet Date: 1978-09-20

Review 2. Linkage map of Salmonella typhimurium, Edition VI.

Authors: K E Sanderson; J R Roth
Journal: Microbiol Rev Date: 1983-09

3. Role for CysJ flavin reductase in molybdenum cofactor-dependent resistance of Escherichia coli to 6-N-hydroxylaminopurine.

Authors: Stanislav G Kozmin; Jian Wang; Roel M Schaaper
Journal: J Bacteriol Date: 2010-01-29 Impact factor: 3.490

4. Constitutive mutation of cysJIH operon in a cysB deletion strain of Salmonella typhimurium.

Authors: J Ostrowski; D Hulanicka
Journal: Mol Gen Genet Date: 1979-09

5. Effect of DNA gyrase inhibitors on gene expression of the cysteine regulon.

Authors: J Ostrowski; D Hulanicka
Journal: Mol Gen Genet Date: 1981

5 in total

Method of isolation of cysteine constitutive mutants of the cysteine regulon in Salmonella typhimurium.

1. Acetylornithinase of Escherichia coli: partial purification and some properties.

2. Determination of serum proteins by means of the biuret reaction.

3. Mutants of Salmonella typhimurium resistant to triazole.

4. Studies on the mechanism of inhibition of Salmonella typhimurium by 1,2,4-triazole.

5. Fine-structure genetic map of the cysB locus in Salmonella typhimurium.

6. A phage P22 gene controlling integration of prophage.

7. Fine-structure mapping and complementation analysis of the Escherichia coli cysB gene.

8. A mutation affecting expression of the gene coding for serine transacetylase in Salmonella typhimurium.