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Literature DB >> 368022

Methylation-dependent DNA synthesis in Escherichia coli mediated by DNA polymerase I.

Abstract

An in vitro system was used to study DNA synthesis in lysates of Escherichia coli cells which had been grown in the presence of ethionine. Such lysates showed a reduced capacity to incorporate [3H]TTP into high-molecular-weight material. Activity could be restored by incubation with S-adenosyl methionine and ATP. S-adenosyl methionine-reactivated TTP incorporation required the presence of DNA polymerase I, ATP, and all four deoxyribonucleotide triphosphates. DNA polymerase III was not required.

Entities: Chemical Species

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Year: 1979 PMID： 368022 PMCID： PMC218416 DOI： 10.1128/jb.137.1.44-50.1979

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

11 in total

1. THE EFFECT OF AMINO ACID DEPRIVATION ON SUBSEQUENT DEOXYRIBONUCLEIC ACID REPLICATION.

Authors: K G LARK; T REPKO; E J HOFFMAN
Journal: Biochim Biophys Acta Date: 1963-09-17

2. Endo R DpnI restriction of Escherichia coli DNA synthesized in vitro. Evidence that the ends of Okazaki pieces are determined by template deoxynucleotide sequence.

Authors: M C Gomez-Eichelmann; K G Lark
Journal: J Mol Biol Date: 1977-12-15 Impact factor: 5.469

3. Deoxyribonucleic acid replication in vitro.

Authors: H Schaller; B Otto; V Nüsslein; J Huf; R Herrmann; F Bonhoeffer
Journal: J Mol Biol Date: 1972-01-28 Impact factor: 5.469

4. Host specificity of DNA produced by Escherichia coli. 13. Breakdown of cellular DNA upon growth in ethionine of strains with r plus-15, r plus-P1 or r plus-N3 restriction phenotypes.

Authors: C Lark; W Arber
Journal: J Mol Biol Date: 1970-09-14 Impact factor: 5.469

10. A conditional lethal mutant of Escherichia coli K12 defective in the 5' leads to 3' exonuclease associated with DNA polymerase I.

Authors: E B Konrad; I R Lehman
Journal: Proc Natl Acad Sci U S A Date: 1974-05 Impact factor: 11.205

3 in total

Methylation-dependent DNA synthesis in Escherichia coli mediated by DNA polymerase I.

1. THE EFFECT OF AMINO ACID DEPRIVATION ON SUBSEQUENT DEOXYRIBONUCLEIC ACID REPLICATION.

2. Endo R DpnI restriction of Escherichia coli DNA synthesized in vitro. Evidence that the ends of Okazaki pieces are determined by template deoxynucleotide sequence.

3. Deoxyribonucleic acid replication in vitro.

4. Host specificity of DNA produced by Escherichia coli. 13. Breakdown of cellular DNA upon growth in ethionine of strains with r plus-15, r plus-P1 or r plus-N3 restriction phenotypes.

5. Replication of Escherichia coli requires DNA polymerase I.

6. Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis.

7. Escherichia coli mutants temperature-sensitive for DNA synthesis.

8. A deoxyribonuclease of Diplococcus pneumoniae specific for methylated DNA.

9. EFFECT OF ETHIONINE ON THE RIBONUCLEIC ACID, DEOXYRIBONUCLEIC ACID, AND PROTEIN CONTENT OF ESCHERICHIA COLI.

10. A conditional lethal mutant of Escherichia coli K12 defective in the 5' leads to 3' exonuclease associated with DNA polymerase I.

1. Modulation of the heat shock response by one-carbon metabolism in Escherichia coli.

Review 2. Bacteriophage T3 and bacteriophage T7 virus-host cell interactions.

Review 3. Molecular aspects of the in vivo and in vitro effects of ethionine, an analog of methionine.