Literature DB >> 3677921

Human chromosome-specific repetitive DNA sequences: novel markers for genetic analysis.

R K Moyzis1, K L Albright, M F Bartholdi, L S Cram, L L Deaven, C E Hildebrand, N E Joste, J L Longmire, J Meyne, T Schwarzacher-Robinson.   

Abstract

Two recombinant DNA clones that are localized to single human chromosomes were isolated from a human repetitive DNA library. Clone pHuR 98, a variant satellite 3 sequence, specifically hybridizes to chromosome position 9qh. Clone pHuR 195, a variant satellite 2 sequence, specifically hybridizes to chromosome position 16qh. These locations were determined by fluorescent in situ hybridization to metaphase chromosomes, and confirmed by DNA hybridizations to human chromosomes sorted by flow cytometry. Pulsed field gel electrophoresis analysis indicated that both sequences exist in the genome as large DNA blocks. In situ hybridization to intact interphase nuclei showed a well-defined, localized organization for both DNA sequences. The ability to tag specific human autosomal chromosomes, both at metaphase and in interphase nuclei, allows novel molecular cytogenetic analyses in numerous basic research and clinical studies.

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Mesh：

Year:  1987        PMID： 3677921     DOI： 10.1007/bf00333988

Source DB:  PubMed          Journal:  Chromosoma        ISSN： 0009-5915            Impact factor:   4.316


  95 in total

Review 1.  Chromosome classification and purification using flow cytometry and sorting.

Authors:  J W Gray; R G Langlois
Journal:  Annu Rev Biophys Biophys Chem       Date:  1986

2.  Organization of a repetitive human 1.8 kb KpnI sequence localized in the heterochromatin of chromosome 15.

Authors:  M J Higgins; H S Wang; I Shtromas; T Haliotis; J C Roder; J J Holden; B N White
Journal:  Chromosoma       Date:  1985       Impact factor: 4.316

Review 3.  Functional aspects of satellite DNA and heterochromatin.

Authors:  B John; G L Miklos
Journal:  Int Rev Cytol       Date:  1979

4.  Gene regulation for higher cells: a theory.

Authors:  R J Britten; E H Davidson
Journal:  Science       Date:  1969-07-25       Impact factor: 47.728

5.  A new method for the preparation of metaphase chromosomes for flow analysis.

Authors:  R Sillar; B D Young
Journal:  J Histochem Cytochem       Date:  1981-01       Impact factor: 2.479

6.  Buoyant density and hybridization analysis of human DNA sequences, including three satellite DNAs.

Authors:  J Prosser; A H Reisner; M L Bradley; K Ho; P C Vincent
Journal:  Biochim Biophys Acta       Date:  1981-11-27

7.  Different central nervous system cell types display distinct and nonrandom arrangements of satellite DNA sequences.

Authors:  L Manuelidis
Journal:  Proc Natl Acad Sci U S A       Date:  1984-05       Impact factor: 11.205

8.  Detection of Y-specific repeat sequences in normal and variant human chromosomes using in situ hybridization with biotinylated probes.

Authors:  Y F Lau
Journal:  Cytogenet Cell Genet       Date:  1985

9.  Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors:  D C Schwartz; C R Cantor
Journal:  Cell       Date:  1984-05       Impact factor: 41.582

10.  Organization and chromosomal specificity of autosomal homologs of human Y chromosome repeated DNA.

Authors:  R D Burk; P Szabo; S O'Brien; W G Nash; L Yu; K D Smith
Journal:  Chromosoma       Date:  1985       Impact factor: 4.316
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  75 in total

1.  Multilocus genetic analysis of single interphase cells by spectral imaging.

Authors:  J Fung; H U Weier; J D Goldberg; R A Pedersen
Journal:  Hum Genet       Date:  2000-12       Impact factor: 4.132

2.  An integrated physical map for the short arm of human chromosome 5.

Authors:  E T Peterson; R Sutherland; D L Robinson; L Chasteen; M Gersh; J Overhauser; L L Deaven; R K Moyzis; D L Grady
Journal:  Genome Res       Date:  1999-12       Impact factor: 9.043

3.  Non-random radial higher-order chromatin arrangements in nuclei of diploid human cells.

Authors:  M Cremer; J von Hase; T Volm; A Brero; G Kreth; J Walter; C Fischer; I Solovei; C Cremer; T Cremer
Journal:  Chromosome Res       Date:  2001       Impact factor: 5.239

4.  A homologous subfamily of satellite III DNA on human chromosomes 14 and 22.

Authors:  K H Choo; E Earle; C McQuillan
Journal:  Nucleic Acids Res       Date:  1990-10-11       Impact factor: 16.971

5.  Intragenomic movement, sequence amplification and concerted evolution in satellite DNA in harvest mice, Reithrodontomys: evidence from in situ hybridization.

Authors:  M J Hamilton; R L Honeycutt; R J Baker
Journal:  Chromosoma       Date:  1990-09       Impact factor: 4.316

6.  Chromosomal origin of small ring marker chromosomes in man: characterization by molecular genetics.

Authors:  D F Callen; H J Eyre; M L Ringenbergs; C J Freemantle; P Woodroffe; E A Haan
Journal:  Am J Hum Genet       Date:  1991-04       Impact factor: 11.025

7.  Detection of aneuploidy involving chromosomes 13, 18, or 21, by fluorescence in situ hybridization (FISH) to interphase and metaphase amniocytes.

Authors:  W L Kuo; H Tenjin; R Segraves; D Pinkel; M S Golbus; J Gray
Journal:  Am J Hum Genet       Date:  1991-07       Impact factor: 11.025

8.  Detection of numerical and structural alterations and fusion of chromosomes 16 and 1 in low-grade papillary breast carcinoma by fluorescence in situ hybridization.

Authors:  H Tsuda; T Takarabe; N Susumu; J Inazawa; S Okada; S Hirohashi
Journal:  Am J Pathol       Date:  1997-10       Impact factor: 4.307

9.  The organisation of repetitive DNA sequences on human chromosomes with respect to the kinetochore analysed using a combination of oligonucleotide primers and CREST anticentromere serum.

Authors:  A Mitchell; P Jeppesen; D Hanratty; J Gosden
Journal:  Chromosoma       Date:  1992-03       Impact factor: 4.316

10.  Cell cycle-dependent recruitment of telomerase RNA and Cajal bodies to human telomeres.

Authors:  Beáta E Jády; Patricia Richard; Edouard Bertrand; Tamás Kiss
Journal:  Mol Biol Cell       Date:  2005-11-30       Impact factor: 4.138
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