Literature DB >> 3674413

An enzyme-linked immunoassay for lipoprotein lipase.

J W Goers1, M E Pedersen, P A Kern, J Ong, M C Schotz.   

Abstract

Polyclonal antibodies against bovine milk lipoprotein lipase (LPL) were used to generate an enzyme-linked immunosorbent assay (ELISA) for rat LPL. The antibodies to LPL were affinity purified on bovine LPL columns and were shown to be specific for LPL by immunoprecipitation and enzyme inhibition. The solid-phase ELISA was sensitive from 1.0 to 20 ng/ml of LPL and paralleled enzyme activity. Denatured rat LPL showed the same LPL mass as undenatured samples, allowing LPL mass to be quantitated effectively in a variety of rat tissue extracts.

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Year:  1987        PMID： 3674413     DOI： 10.1016/0003-2697(87)90541-0

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  15 in total

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4.  Regulation of lipoprotein lipase translation by epinephrine in 3T3-L1 cells. Importance of the 3' untranslated region.

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9.  Regulation of lipoprotein lipase immunoreactive mass in isolated human adipocytes.

Authors:  P A Kern; J M Ong; J W Goers; M E Pedersen
Journal:  J Clin Invest       Date:  1988-02       Impact factor: 14.808

10.  The interaction of Bacteroides fragilis with components of the human fibrinolytic system.

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