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Literature DB >> 3671067

Adenine specific DNA chemical sequencing reaction.

Abstract

Reaction of DNA with K2PdCl4 at pH 2.0 followed by a piperidine workup produces specific cleavage at adenine (A) residues. Product analysis revealed the K2PdCl4 reaction involves selective depurination at adenine, affording an excision reaction analogous to the other chemical DNA sequencing reactions. Adenine residues methylated at the exocyclic amine (N6) react with lower efficiency than unmethylated adenine in an identical sequence. This simple protocol specific for A may be a useful addition to current chemical sequencing reactions.

Entities: Chemical

Mesh：

Substances：

Year: 1987 PMID： 3671067 PMCID： PMC306310 DOI： 10.1093/nar/15.19.7823

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

6 in total

1. Base-specific reactions useful for DNA sequencing: methylene blue--sensitized photooxidation of guanine and osmium tetraoxide modification of thymine.

Authors: T Friedmann; D M Brown
Journal: Nucleic Acids Res Date: 1978-02 Impact factor: 16.971

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Authors: A M Maxam; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

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Authors: A M Maxam; W Gilbert
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

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Authors: B J Ambrose; R C Pless
Journal: Biochemistry Date: 1985-10-22 Impact factor: 3.162

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Authors: N Okawa; Y Suyama; A Kaji
Journal: Nucleic Acids Res Date: 1985-11-11 Impact factor: 16.971

6. Map of distamycin, netropsin, and actinomycin binding sites on heterogeneous DNA: DNA cleavage-inhibition patterns with methidiumpropyl-EDTA.Fe(II).

Authors: M W Van Dyke; R P Hertzberg; P B Dervan
Journal: Proc Natl Acad Sci U S A Date: 1982-09 Impact factor: 11.205

6 in total

29 in total

1. Optimal conditions to use Pfu exo(-) DNA polymerase for highly efficient ligation-mediated polymerase chain reaction protocols.

Authors: M Angers; J F Cloutier; A Castonguay; R Drouin
Journal: Nucleic Acids Res Date: 2001-08-15 Impact factor: 16.971

2. Inhibition of heat shock transcription factor binding by a linear polyamide binding in an unusual 1:1 mode.

Authors: Rongsheng E Wang; Raj K Pandita; Jianfeng Cai; Clayton R Hunt; John-Stephen Taylor
Journal: Chembiochem Date: 2011-12-01 Impact factor: 3.164

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Authors: M Nelson; J L Van Etten; R Grabherr
Journal: Nucleic Acids Res Date: 1992-03-25 Impact factor: 16.971

4. The influence of single base triplet changes on the stability of a pur.pur.pyr triple helix determined by affinity cleaving.

Authors: P A Beal; P B Dervan
Journal: Nucleic Acids Res Date: 1992-06-11 Impact factor: 16.971

5. Orientation of the Lac repressor DNA binding domain in complex with the left lac operator half site characterized by affinity cleaving.

Authors: J A Shin; R H Ebright; P B Dervan
Journal: Nucleic Acids Res Date: 1991-10-11 Impact factor: 16.971

6. A Solution Phase Platform to Characterize Chemical Reaction Compatibility with DNA-Encoded Chemical Library Synthesis.

Authors: Anokha S Ratnayake; Mark E Flanagan; Timothy L Foley; Justin D Smith; Jillian G Johnson; Justin Bellenger; Justin I Montgomery; Brian M Paegel
Journal: ACS Comb Sci Date: 2019-09-06 Impact factor: 3.784

10. Polyamine effects on purine-purine-pyrimidine triple helix formation by phosphodiester and phosphorothioate oligodeoxyribonucleotides.

Authors: M Musso; M W Van Dyke
Journal: Nucleic Acids Res Date: 1995-06-25 Impact factor: 16.971