Literature DB >> 3663168

The separation of glutathione transferase subunits by using reverse-phase high-pressure liquid chromatography.

A K Ostlund Farrants1, D J Meyer, B Coles, C Southan, A Aitken, P J Johnson, B Ketterer.   

Abstract

A simple method is described for the separation and quantification of the subunits of GSH transferases present in rat tissue extracts. This method, involving GSH-agarose affinity chromatography followed by reverse-phase h.p.l.c., is rapid and sufficiently sensitive to measure 5 micrograms of each subunit in a mixture. Examples are given of its application to extracts of rat kidney, adrenal, testicular interstitial cells and seminiferous tubules. The analysis of seminiferous tubules indicates that the technique may be of value for the identification of novel subunits. Preliminary separations of subunits from human GSH transferases are also described.

Entities:  

Mesh：

Substances：

Year:  1987        PMID： 3663168      PMCID： PMC1148139          DOI： 10.1042/bj2450423

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  26 in total

1.  The environment of function of liver and red blood cells.

Authors:  D L Drabkin
Journal:  Ann N Y Acad Sci       Date:  1975-04-15       Impact factor: 5.691

2.  Radioimmunoassay of ligandin.

Authors:  N M Bass; R E Kirsch; S A Tuff; S J Saunders
Journal:  Biochim Biophys Acta       Date:  1977-09-27

3.  Conversion of cholesterol to androgens by rat testes: comparison of interstitial cells and seminiferous tubules.

Authors:  P F Hall; D C Irby; D M De Kretser
Journal:  Endocrinology       Date:  1969-03       Impact factor: 4.736

4.  Differential tissue expression of the glutathione transferase multigene family.

Authors:  S E Pemble; J B Taylor; R K Craig; B Ketterer
Journal:  Biochem J       Date:  1986-09-01       Impact factor: 3.857

5.  Evidence that the Ya and Yc subunits of glutathione transferase B (ligandin) are the products of separate genes.

Authors:  D Beale; B Ketterer; T Carne; D Meyer; J B Taylor
Journal:  Eur J Biochem       Date:  1982-09-01

6.  Purification and characterisation of prostaglandin endoperoxide D-isomerase, a cytoplasmic, glutathione-requiring enzyme.

Authors:  E Christ-Hazelhof; D H Nugteren
Journal:  Biochim Biophys Acta       Date:  1979-01-29

7.  Rat liver glutathione S-transferases. Complete nucleotide sequence of a glutathione S-transferase mRNA and the regulation of the Ya, Yb, and Yc mRNAs by 3-methylcholanthrene and phenobarbital.

Authors:  C B Pickett; C A Telakowski-Hopkins; G J Ding; L Argenbright; A Y Lu
Journal:  J Biol Chem       Date:  1984-04-25       Impact factor: 5.157

8.  Evidence that the Yb subunits of hepatic glutathione transferases represent two different but related families of polypeptides.

Authors:  D Beale; D J Meyer; J B Taylor; B Ketterer
Journal:  Eur J Biochem       Date:  1983-12-01

9.  The influence of soluble binding proteins on lipophile transport and metabolism in hepatocytes.

Authors:  E Tipping; B Ketterer
Journal:  Biochem J       Date:  1981-05-01       Impact factor: 3.857

10.  The nucleotide sequence of a rat liver glutathione S-transferase subunit cDNA clone.

Authors:  H C Lai; N Li; M J Weiss; C C Reddy; C P Tu
Journal:  J Biol Chem       Date:  1984-05-10       Impact factor: 5.157
View more
  51 in total

1.  Quantitative profiling of tissue- and gender-related expression of glutathione S-transferase isoenzymes in the mouse.

Authors:  A E Mitchell; D Morin; J Lakritz; A D Jones
Journal:  Biochem J       Date:  1997-07-01       Impact factor: 3.857

2.  Glutathione S-transferase pi in an arsenic-resistant Chinese hamster ovary cell line.

Authors:  J F Lo; H F Wang; M F Tam; T C Lee
Journal:  Biochem J       Date:  1992-12-15       Impact factor: 3.857

3.  Cloning, sequencing and characterization of the human alpha glutathione S-transferase gene corresponding to the cDNA clone pGTH2.

Authors:  A Klöne; R Hussnätter; H Sies
Journal:  Biochem J       Date:  1992-08-01       Impact factor: 3.857

4.  Tissue distribution of enzymic methylation of glutathione S-transferase and its effects on catalytic activity. Methylation of glutathione S-transferase 11-11 inhibits conjugating activity towards 1-chloro-2,4-dinitrobenzene.

Authors:  J A Johnson; K A Finn; F L Siegel
Journal:  Biochem J       Date:  1992-02-15       Impact factor: 3.857

5.  Design of two chimaeric human-rat class alpha glutathione transferases for probing the contribution of C-terminal segments of protein structure to the catalytic properties.

Authors:  R Björnestedt; M Widersten; P G Board; B Mannervik
Journal:  Biochem J       Date:  1992-03-01       Impact factor: 3.857

6.  Glutathione transferase isoenzymes from Bufo bufo embryos at an early developmental stage.

Authors:  C Di Ilio; A Aceto; T Bucciarelli; B Dragani; S Angelucci; M Miranda; A Poma; F Amicarelli; D Barra; G Federici
Journal:  Biochem J       Date:  1992-04-01       Impact factor: 3.857

7.  Participation of the phenolic hydroxyl group of Tyr-8 in the catalytic mechanism of human glutathione transferase P1-1.

Authors:  R H Kolm; G E Sroga; B Mannervik
Journal:  Biochem J       Date:  1992-07-15       Impact factor: 3.857

8.  Effects of hyperbilirubinaemia on glutathione S-transferase isoenzymes in cerebellar cortex of the Gunn rat.

Authors:  J A Johnson; J J Hayward; S E Kornguth; F L Siegel
Journal:  Biochem J       Date:  1993-04-15       Impact factor: 3.857

9.  Constitutive and inducible profile of glutathione S-transferase subunits in biliary epithelial cells and hepatocytes isolated from rat liver.

Authors:  M Parola; M E Biocca; G Leonarduzzi; E Albano; M U Dianzani; K S Gilmore; D J Meyer; B Ketterer; T F Slater; K H Cheeseman
Journal:  Biochem J       Date:  1993-04-15       Impact factor: 3.857

10.  Expression of glutathione S-transferase during rat liver development.

Authors:  L B Tee; K S Gilmore; D J Meyer; B Ketterer; Y Vandenberghe; G C Yeoh
Journal:  Biochem J       Date:  1992-02-15       Impact factor: 3.857
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.