Literature DB >> 3632693

Isolation of murine pluripotent hemopoietic stem cells in the Go phase.

M Miyama-Inaba, H Ogata, J Toki, S Kuma, K Sugiura, R Yasumizu, S Ikehara.   

Abstract

A method to purify pluripotent hemopoietic stem cells in the Go phase from mouse bone marrow was established. Bone marrow cells from 5-fluorouracil (5-FU)-treated mice were fractionated by Percoll density gradient. The cells with density between 1.063 and 1.075 were further separated into wheat germ agglutinin (WGA)-positive and -negative cells using fluorescent-activated cell sorter (FACS) after staining with fluorescein isothiocyanate-conjugated WGA (FITC-WGA). An assay for spleen colony-forming units (CFU-S) revealed that the WGA-positive cells (1 X 10(6)) produced 1380 CFU-S (about 150 times of the number in the original bone marrow cells) on day 12 (but no CFU-S on day 8), whereas the WGA-negative cells produced no CFU-S. Thus, the stem cells in the Go phase are found to be enriched 150 times in 5-FU-treated WGA-positive cells.

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Year:  1987        PMID： 3632693     DOI： 10.1016/0006-291x(87)90985-5

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  7 in total

1.  Organ-specific and systemic autoimmune diseases originate from defects in hematopoietic stem cells.

Authors:  S Ikehara; M Kawamura; F Takao; M Inaba; R Yasumizu; S Than; H Hisha; K Sugiura; Y Koide; T O Yoshida
Journal:  Proc Natl Acad Sci U S A       Date:  1990-11       Impact factor: 11.205

2.  Transplantation of wheat germ agglutinin-positive hematopoietic cells to prevent or induce systemic autoimmune disease.

Authors:  E E Sardiña; K Sugiura; S Ikehara; R A Good
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-15       Impact factor: 11.205

3.  Pluripotent hemopoietic stem cells are c-kit<low.

Authors:  H Doi; M Inaba; Y Yamamoto; S Taketani; S I Mori; A Sugihara; H Ogata; J Toki; H Hisha; K Inaba; S Sogo; M Adachi; T Matsuda; R A Good; S Ikehara
Journal:  Proc Natl Acad Sci U S A       Date:  1997-03-18       Impact factor: 11.205

4.  Long-term repopulation of hematolymphoid cells with only a few hemopoietic stem cells in mice.

Authors:  H Ogata; W G Bradley; M Inaba; N Ogata; S Ikehara; R A Good
Journal:  Proc Natl Acad Sci U S A       Date:  1995-06-20       Impact factor: 11.205

5.  Analyses of T-cell differentiation from hemopoietic stem cells in the G0 phase by an in vitro method.

Authors:  J Toki; T Kumamoto; H Ogata; M Kawamura; M Fukumoto; Y Yamamoto; S Than; M Inaba; Y Himeno
Journal:  Proc Natl Acad Sci U S A       Date:  1991-09-01       Impact factor: 11.205

6.  Lymphohemopoietic reconstitution using wheat germ agglutinin-positive hemopoietic stem cell transplantation within but not across the major histocompatibility antigen barriers.

Authors:  N S el Badri; R A Good
Journal:  Proc Natl Acad Sci U S A       Date:  1993-07-15       Impact factor: 11.205

7.  Focal segmental glomerular sclerosis, a type of intractable chronic glomerulonephritis, is a stem cell disorder.

Authors:  M Nishimura; J Toki; K Sugiura; F Hashimoto; T Tomita; H Fujishima; Y Hiramatsu; N Nishioka; N Nagata; Y Takahashi
Journal:  J Exp Med       Date:  1994-03-01       Impact factor: 14.307
  7 in total

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