Literature DB >> 36272070

Hybridization Chain Reaction Fluorescence In Situ Hybridization (HCR-FISH) in Ambystoma mexicanum Tissue.

Alex M Lovely1,2, Timothy J Duerr1, David F Stein1, Evan T Mun1, James R Monaghan3,4.   

Abstract

In situ hybridization is a standard procedure for visualizing mRNA transcripts in tissues. The recent adoption of fluorescent probes and new signal amplification methods have facilitated multiplexed RNA imaging in tissue sections and whole tissues. Here we present protocols for multiplexed hybridization chain reaction fluorescence in situ hybridization (HCR-FISH) staining, imaging, cell segmentation, and mRNA quantification in regenerating axolotl tissue sections. We also present a protocol for whole-mount staining and imaging of developing axolotl limbs.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Axolotl; Hybridization chain reaction; In situ hybridization; Limb development; Limb regeneration

Year:  2023        PMID： 36272070     DOI： 10.1007/978-1-0716-2659-7_6

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  28 in total

1.  RNAscope: a novel in situ RNA analysis platform for formalin-fixed, paraffin-embedded tissues.

Authors:  Fay Wang; John Flanagan; Nan Su; Li-Chong Wang; Son Bui; Allissa Nielson; Xingyong Wu; Hong-Thuy Vo; Xiao-Jun Ma; Yuling Luo
Journal:  J Mol Diagn       Date:  2012-01       Impact factor: 5.568

2.  In situ hybridization of slow myosin heavy chain mRNA in normal and transforming rabbit muscles with the use of a nonradioactively labeled cRNA.

Authors:  S Aigner; D Pette
Journal:  Histochemistry       Date:  1990

3.  Spatial organization of the somatosensory cortex revealed by osmFISH.

Authors:  Simone Codeluppi; Lars E Borm; Amit Zeisel; Gioele La Manno; Josina A van Lunteren; Camilla I Svensson; Sten Linnarsson
Journal:  Nat Methods       Date:  2018-10-30       Impact factor: 28.547

4.  Formation and detection of RNA-DNA hybrid molecules in cytological preparations.

Authors:  J G Gall; M L Pardue
Journal:  Proc Natl Acad Sci U S A       Date:  1969-06       Impact factor: 11.205

5.  RNA-DNA hybrids at the cytological level.

Authors:  H A John; M L Birnstiel; K W Jones
Journal:  Nature       Date:  1969-08-09       Impact factor: 49.962

6.  Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.

Authors:  D A Melton; P A Krieg; M R Rebagliati; T Maniatis; K Zinn; M R Green
Journal:  Nucleic Acids Res       Date:  1984-09-25       Impact factor: 16.971

7.  A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback.

Authors:  D Tautz; C Pfeifle
Journal:  Chromosoma       Date:  1989-08       Impact factor: 4.316

8.  Interphase cytogenetics using biotin and digoxigenin labelled probes I: relative sensitivity of both reporter molecules for detection of HPV16 in CaSki cells.

Authors:  C S Herrington; J Burns; A K Graham; M Evans; J O McGee
Journal:  J Clin Pathol       Date:  1989-06       Impact factor: 3.411

9.  Comparison of 35S- and digoxigenin-labeled RNA and oligonucleotide probes for in situ hybridization. Expression of mRNA of the seminal vesicle secretion protein II and androgen receptor genes in the rat prostate.

Authors:  P Komminoth; F B Merk; I Leav; H J Wolfe; J Roth
Journal:  Histochemistry       Date:  1992-11

10.  Imaging individual mRNA molecules using multiple singly labeled probes.

Authors:  Arjun Raj; Patrick van den Bogaard; Scott A Rifkin; Alexander van Oudenaarden; Sanjay Tyagi
Journal:  Nat Methods       Date:  2008-09-21       Impact factor: 28.547
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