Aida Doostkam1, Hossein Mirkhani2, Kamyar Iravani3, Saied Karbalay-Doust4, Afsaneh Doosti5, Elham Nadimi6, Fatema Pirsalami7. 1. Shiraz Nephro-Urology Research Center, Shiraz University of Medical Sciences, Shiraz 7193635899, Iran. 2. Department of Pharmacology, School of Medicine, Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz 7134853185, Iran. 3. Department of Otolaryngology, Otolaryngology Research Center, Shiraz University of Medical Sciences, Shiraz 71936-16641, Iran. 4. Department of Anatomy, School of Medicine, Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz 7134853185, Iran. 5. Department of Audiology, School of Rehabilitation Sciences, Rehabilitation Sciences Research Center, Shiraz University of Medical Sciences, Shiraz 7194733669, Iran. 6. Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz 7134853185, Iran. 7. Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz 7134853185, Iran.
Abstract
OBJECTIVE: Diabetic auditory neuropathy (DAN) is a common complication of diabetes that seriously affects the quality of life in patients. In this study, we investigate the role of folic acid in the treatment of DAN in an experimental rat model. METHODS: Thirty-two Sprague-Dawley rats were equally divided into four groups: group 1, normal; group 2, diabetic rats; and groups 3 and 4, diabetic rats treated with folic acid (40 and 80 mg/kg, respectively). We used some tools to investigate the therapeutic effect of folic acid on DAN. We evaluated auditory brain stem response (ABR), estimated the volume and number of spiral ganglion and the volume of stria vascularis and spiral ligament by the stereological method, and measured the blood levels of homocysteine (HCY), malondialdehyde (MDA), and superoxide dismutase (SOD). RESULTS: Our study showed that folic acid treatment was not significantly effective in improving structural and functional disorders in DAN, even though its effectiveness in reducing HCY (P < 0.001) and MDA (P < 0.05) as oxidative biomarkers was significant. CONCLUSION: Folic acid is not effective in relieving morphological and functional disorders in DAN.
OBJECTIVE: Diabetic auditory neuropathy (DAN) is a common complication of diabetes that seriously affects the quality of life in patients. In this study, we investigate the role of folic acid in the treatment of DAN in an experimental rat model. METHODS: Thirty-two Sprague-Dawley rats were equally divided into four groups: group 1, normal; group 2, diabetic rats; and groups 3 and 4, diabetic rats treated with folic acid (40 and 80 mg/kg, respectively). We used some tools to investigate the therapeutic effect of folic acid on DAN. We evaluated auditory brain stem response (ABR), estimated the volume and number of spiral ganglion and the volume of stria vascularis and spiral ligament by the stereological method, and measured the blood levels of homocysteine (HCY), malondialdehyde (MDA), and superoxide dismutase (SOD). RESULTS: Our study showed that folic acid treatment was not significantly effective in improving structural and functional disorders in DAN, even though its effectiveness in reducing HCY (P < 0.001) and MDA (P < 0.05) as oxidative biomarkers was significant. CONCLUSION: Folic acid is not effective in relieving morphological and functional disorders in DAN.
Authors: Juan Carlos Alvarado; Verónica Fuentes-Santamaría; Tania Jareño-Flores; José Luis Blanco; José M Juiz Journal: Neurosci Res Date: 2012-05-14 Impact factor: 3.304
Authors: Eva L Feldman; Brian C Callaghan; Rodica Pop-Busui; Douglas W Zochodne; Douglas E Wright; David L Bennett; Vera Bril; James W Russell; Vijay Viswanathan Journal: Nat Rev Dis Primers Date: 2019-06-13 Impact factor: 52.329