Dulyapong Rungraungrayabkul1, Puangwan Lapthanasupkul2, Nakarin Kitkumthorn3, Jintana Pankam4, Boworn Klongnoi4,5, Siribang-On Piboonniyom Khovidhunkit6,7. 1. Department of Oral Medicine and Periodontology, Faculty of Dentistry, Mahidol University, Rajthewee, Bangkok, 10400, Thailand. 2. Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mahidol University, Rajthewee, Bangkok, 10400, Thailand. 3. Department of Oral Biology, Faculty of Dentistry, Mahidol University, Rajthewee, Bangkok, 10400, Thailand. 4. Development of Disease Management Model for Oral Cancer With an Integration Network of Screening, Surveillance, and Treatment From Primary Care Unit to Tertiary Care in Nakhonratchasima Province Project, Faculty of Dentistry, Mahidol University, Rajthewee, Bangkok, 10400, Thailand. 5. Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Mahidol University, Rajthewee, Bangkok, 10400, Thailand. 6. Development of Disease Management Model for Oral Cancer With an Integration Network of Screening, Surveillance, and Treatment From Primary Care Unit to Tertiary Care in Nakhonratchasima Province Project, Faculty of Dentistry, Mahidol University, Rajthewee, Bangkok, 10400, Thailand. Siribangon.pib@mahidol.edu. 7. Department of Advanced General Dentistry, Faculty of Dentistry, Mahidol University, Rajthewee, Bangkok, 10400, Thailand. Siribangon.pib@mahidol.edu.
Abstract
BACKGROUND: To date, high-risk human papillomavirus (HPV) is primarily linked to oropharyngeal carcinoma, but only a small subset of oral squamous cell carcinoma (OSCC) is truly driven by high-risk HPV. In addition, Epstein-Barr virus (EBV) is another potential oncogenic virus for OSCC development. This study aims to investigate the role of EBV infection in Thai patients with OSCC. METHODS: Forty-seven formalin-fixed paraffin-embedded specimens of OSCC were obtained. EBV DNA was detected by polymerase chain reaction analysis using primers for LMP-1 region of EBV. EBV-positive OSCC cases were subjected to LMP-1 immunohistochemical analysis and EBV-encoded small RNA (EBER) in situ hybridization to determine EBV cellular localization in OSCC. LMP-1 immunohistochemical analysis was also performed in all EBV-negative OSCC cases. RESULTS: Of the 47 OSCC specimens, ten (21%) exhibited EBV DNA by PCR analysis. Seven of ten (70%) EBV-positive specimens showed high-grade LMP-1 expression by immunohistochemistry. However, no EBER expression was detected in all EBV-positive OSCC specimens. In EBV-negative specimens, LMP-1 was also negative except in 3 specimens which showed low grade expression of LMP-1. CONCLUSION: The prevalence of EBV infection in OSCC in this group of Thai patients was 21%. Most of EBV-positive OSCC cases showed LMP-1 expression but a lack of EBER expression. From our findings, we presume that EBV may take some roles in OSCC development in this group of participants.
BACKGROUND: To date, high-risk human papillomavirus (HPV) is primarily linked to oropharyngeal carcinoma, but only a small subset of oral squamous cell carcinoma (OSCC) is truly driven by high-risk HPV. In addition, Epstein-Barr virus (EBV) is another potential oncogenic virus for OSCC development. This study aims to investigate the role of EBV infection in Thai patients with OSCC. METHODS: Forty-seven formalin-fixed paraffin-embedded specimens of OSCC were obtained. EBV DNA was detected by polymerase chain reaction analysis using primers for LMP-1 region of EBV. EBV-positive OSCC cases were subjected to LMP-1 immunohistochemical analysis and EBV-encoded small RNA (EBER) in situ hybridization to determine EBV cellular localization in OSCC. LMP-1 immunohistochemical analysis was also performed in all EBV-negative OSCC cases. RESULTS: Of the 47 OSCC specimens, ten (21%) exhibited EBV DNA by PCR analysis. Seven of ten (70%) EBV-positive specimens showed high-grade LMP-1 expression by immunohistochemistry. However, no EBER expression was detected in all EBV-positive OSCC specimens. In EBV-negative specimens, LMP-1 was also negative except in 3 specimens which showed low grade expression of LMP-1. CONCLUSION: The prevalence of EBV infection in OSCC in this group of Thai patients was 21%. Most of EBV-positive OSCC cases showed LMP-1 expression but a lack of EBER expression. From our findings, we presume that EBV may take some roles in OSCC development in this group of participants.
Authors: Jamshid Jalouli; Salah O Ibrahim; Ravi Mehrotra; Miranda M Jalouli; Dipak Sapkota; Per-Anders Larsson; Jan-M Hirsch Journal: Acta Otolaryngol Date: 2010-11 Impact factor: 1.494
Authors: Marcos Antonio Pereira de Lima; Roberta Barroso Cavalcante; Cláudio Gleidiston Lima da Silva; Renato Luiz Maia Nogueira; Geamberg Einstein Cruz Macedo; Lara Eduardo de Galiza; Juliana Viana Pinheiro; Pedro Hugo Bezerra Maia Filho; Sarah Ferreira Santos; Silvia Helena Barem Rabenhorst Journal: Oral Dis Date: 2021-03-19 Impact factor: 3.511