Literature DB >> 36227477

Determining Thermodynamic and Material Properties of Biomolecular Condensates by Confocal Microscopy and Optical Tweezers.

Archishman Ghosh1, Divya Kota1, Huan-Xiang Zhou2,3.   

Abstract

While the roles of biomolecular condensates in health and disease are being intensely studied, it is equally important that their physical properties are characterized in order to achieve mechanistic understanding. Here we share some of the protocols developed in our lab for measuring thermodynamic and materials properties of condensates. These include a simple method for determining the droplet-phase concentrations of condensate components on a confocal microscope, and a method for determining the viscoelasticity of condensates by optical tweezers. These protocols are either generally applicable to biomolecular condensates or are unique for their characterization.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Biomolecular condensates; Confocal microscopy; Fusion speed; Interfacial tension; Optical tweezers; Viscoelasticity

Mesh：

Year:  2023        PMID： 36227477      PMCID： PMC9577454          DOI： 10.1007/978-1-0716-2663-4_12

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  24 in total

1.  Tug of War between Condensate Phases in a Minimal Macromolecular System.

Authors:  Archishman Ghosh; Xiaojia Zhang; Huan-Xiang Zhou
Journal:  J Am Chem Soc       Date:  2020-05-04       Impact factor: 15.419

2.  The disordered P granule protein LAF-1 drives phase separation into droplets with tunable viscosity and dynamics.

Authors:  Shana Elbaum-Garfinkle; Younghoon Kim; Krzysztof Szczepaniak; Carlos Chih-Hsiung Chen; Christian R Eckmann; Sua Myong; Clifford P Brangwynne
Journal:  Proc Natl Acad Sci U S A       Date:  2015-05-26       Impact factor: 11.205

Review 3.  Liquid-Liquid Phase Separation in Disease.

Authors:  Simon Alberti; Dorothee Dormann
Journal:  Annu Rev Genet       Date:  2019-08-20       Impact factor: 16.830

4.  Protein condensates as aging Maxwell fluids.

Authors:  Louise Jawerth; Elisabeth Fischer-Friedrich; Suropriya Saha; Jie Wang; Titus Franzmann; Xiaojie Zhang; Jenny Sachweh; Martine Ruer; Mahdiye Ijavi; Shambaditya Saha; Julia Mahamid; Anthony A Hyman; Frank Jülicher
Journal:  Science       Date:  2020-12-11       Impact factor: 47.728

5.  Determinants for Fusion Speed of Biomolecular Droplets.

Authors:  Archishman Ghosh; Huan-Xiang Zhou
Journal:  Angew Chem Int Ed Engl       Date:  2020-09-08       Impact factor: 15.336

6.  Local measurements of viscoelastic moduli of entangled actin networks using an oscillating magnetic bead micro-rheometer.

Authors:  F Ziemann; J Rädler; E Sackmann
Journal:  Biophys J       Date:  1994-06       Impact factor: 4.033

7.  Coexisting Liquid Phases Underlie Nucleolar Subcompartments.

Authors:  Marina Feric; Nilesh Vaidya; Tyler S Harmon; Diana M Mitrea; Lian Zhu; Tiffany M Richardson; Richard W Kriwacki; Rohit V Pappu; Clifford P Brangwynne
Journal:  Cell       Date:  2016-05-19       Impact factor: 41.582

8.  Programmable viscoelasticity in protein-RNA condensates with disordered sticker-spacer polypeptides.

Authors:  Ibraheem Alshareedah; Mahdi Muhammad Moosa; Matthew Pham; Davit A Potoyan; Priya R Banerjee
Journal:  Nat Commun       Date:  2021-11-16       Impact factor: 14.919

9.  Free mRNA in excess upon polysome dissociation is a scaffold for protein multimerization to form stress granules.

Authors:  Ouissame Bounedjah; Bénédicte Desforges; Ting-Di Wu; Catherine Pioche-Durieu; Sergio Marco; Loic Hamon; Patrick A Curmi; Jean-Luc Guerquin-Kern; Olivier Piétrement; David Pastré
Journal:  Nucleic Acids Res       Date:  2014-07-10       Impact factor: 16.971
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