Literature DB >> 36227473

Fluorescence Correlation Spectroscopy and Phase Separation.

Juan Jeremías Incicco1,2, Debjit Roy1,2, Melissa D Stuchell-Brereton1,2, Andrea Soranno3,4.   

Abstract

A quantitative understanding of the forces controlling the assembly and functioning of biomolecular condensates requires the identification of phase boundaries at which condensates form as well as the determination of tie-lines. Here, we describe in detail how Fluorescence Correlation Spectroscopy (FCS) provides a versatile approach to estimate phase boundaries of single-component and multicomponent solutions as well as insights about the transport properties of the condensate.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Biomolecular condensates; Fluorescence Correlation Spectroscopy; Intrinsically disordered proteins; Phase separation

Mesh：

Year:  2023        PMID： 36227473     DOI： 10.1007/978-1-0716-2663-4_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  50 in total

1.  Germline P granules are liquid droplets that localize by controlled dissolution/condensation.

Authors:  Clifford P Brangwynne; Christian R Eckmann; David S Courson; Agata Rybarska; Carsten Hoege; Jöbin Gharakhani; Frank Jülicher; Anthony A Hyman
Journal:  Science       Date:  2009-05-21       Impact factor: 47.728

Review 2.  Recent Developments in the Field of Intrinsically Disordered Proteins: Intrinsic Disorder-Based Emergence in Cellular Biology in Light of the Physiological and Pathological Liquid-Liquid Phase Transitions.

Authors:  Vladimir N Uversky
Journal:  Annu Rev Biophys       Date:  2021-01-27       Impact factor: 12.981

Review 3.  Who's In and Who's Out-Compositional Control of Biomolecular Condensates.

Authors:  Jonathon A Ditlev; Lindsay B Case; Michael K Rosen
Journal:  J Mol Biol       Date:  2018-08-09       Impact factor: 5.469

4.  Determinants for Fusion Speed of Biomolecular Droplets.

Authors:  Archishman Ghosh; Huan-Xiang Zhou
Journal:  Angew Chem Int Ed Engl       Date:  2020-09-08       Impact factor: 15.336

5.  Phase transition of RNA-protein complexes into ordered hollow condensates.

Authors:  Ibraheem Alshareedah; Mahdi Muhammad Moosa; Muralikrishna Raju; Davit A Potoyan; Priya R Banerjee
Journal:  Proc Natl Acad Sci U S A       Date:  2020-06-22       Impact factor: 11.205

6.  Quantifying viscosity and surface tension of multicomponent protein-nucleic acid condensates.

Authors:  Ibraheem Alshareedah; George M Thurston; Priya R Banerjee
Journal:  Biophys J       Date:  2021-01-14       Impact factor: 4.033

7.  Phase transitions in the assembly of multivalent signalling proteins.

Authors:  Pilong Li; Sudeep Banjade; Hui-Chun Cheng; Soyeon Kim; Baoyu Chen; Liang Guo; Marc Llaguno; Javoris V Hollingsworth; David S King; Salman F Banani; Paul S Russo; Qiu-Xing Jiang; B Tracy Nixon; Michael K Rosen
Journal:  Nature       Date:  2012-03-07       Impact factor: 49.962

8.  Programmable viscoelasticity in protein-RNA condensates with disordered sticker-spacer polypeptides.

Authors:  Ibraheem Alshareedah; Mahdi Muhammad Moosa; Matthew Pham; Davit A Potoyan; Priya R Banerjee
Journal:  Nat Commun       Date:  2021-11-16       Impact factor: 14.919

Review 9.  Biomolecular condensates: organizers of cellular biochemistry.

Authors:  Salman F Banani; Hyun O Lee; Anthony A Hyman; Michael K Rosen
Journal:  Nat Rev Mol Cell Biol       Date:  2017-02-22       Impact factor: 94.444
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