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Literature DB >> 362155

A DNA segment within the colicin E1 structural gene on ColE1 affecting immunity to colicin.

Abstract

Insertion of DNA at the EcoRI site of ColE1 results in increase of immunity to colicin killing in E. coli harboring such recombinant ColE1 plasmid as compared to E. coli (ColE1). This effect is neither due to cis or trans interactions originating from the inserted foreign DNA fragment, nor to changes in plasmid copy number. This defect in the immunity mechanism is not trans complemented for by wild type ColE1. Increase in immunity can also be obtained by deleting a DNA segment from the ColE1 genome. This segment is approximately 120 bp left to the EcoRI site within the colicin structural gene. It is concluded that the structure of DNA per se, around the EcoRI site, within colcin structural gene, is the structure which affects immunity expression.

Entities: Species

Mesh：

Substances：
Colicins
DNA, Bacterial

Year: 1978 PMID： 362155 DOI： 10.1007/bf00333155

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

16 in total

A DNA segment within the colicin E1 structural gene on ColE1 affecting immunity to colicin.

1. New features of the regulation of the tryptophan operon.

2. Colicins and colicinogenic factors.

3. Studies of colicin E1 plasmid functions by analysis of deletions and TnA insertions of the plasmid.

4. Construction of biologically functional bacterial plasmids in vitro.

5. Purification and properties of colicin E3 immunity protein.

6. E3 immunity substance. A protein from e3-colicinogenic cells that accounts for their immunity to colicin E3.

7. Tetracycline resistance expressed by pSC101 hybrids.

8. Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid.

9. The transposon Tn1 as a probe for studying ColE1 structure and function.

10. Protein expression in E. coli minicells by recombinant plasmids.

1. ColE1 DNA sequences interacting in cis, essential for mitomycin-C induced lethality.

2. Analysis of ColE1 expression in vitro after chromosome fragmentation.

3. Plasmid-determined immunity of Escherichia coli K-12 to colicin Ia Is mediated by a plasmid-encoded membrane protein.

4. Mitomycin-induced lethality of Escherichia coli cells containing the ColE1 Plasmid: involvement of the kil gene.

5. Regulation of dihydrofolate reductase synthesis in Escherichia coli.

6. Localization of genes responsible for replication and immunity to colicin A on plasmid ColA-CA31.