Literature DB >> 36180636

Regulatory T-Cell Phenotyping Using CyTOF.

Hugo Barcenilla1, Mikael Pihl2, Florence Sjögren2, Louise Magnusson3, Rosaura Casas3.   

Abstract

Regulatory T cells are an important component of the immune system that plays a key role in maintaining homeostasis. Identification of distinct regulatory T cell subsets is essential to understand their function. Mass cytometry or CyTOF is a technology that enables the simultaneous measurement of up to 50 markers in single cells by using antibodies tagged with heavy metals, which are then detected with time-of-flight mass spectrometry. This chapter describes a mass cytometry approach for phenotypic characterization of regulatory T cells and determination of their master transcription factor Foxp3.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  CyTOF; Foxp3; Mass cytometry; Regulatory T cells; Single-cell analysis; Treg; immunophenotyping; phenotyping

Mesh：

Substances：

Year:  2023        PMID： 36180636     DOI： 10.1007/978-1-0716-2647-4_15

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  18 in total

1.  Functionally distinct subsets of human FOXP3+ Treg cells that phenotypically mirror effector Th cells.

Authors:  Thomas Duhen; Rebekka Duhen; Antonio Lanzavecchia; Federica Sallusto; Daniel J Campbell
Journal:  Blood       Date:  2012-03-21       Impact factor: 22.113

2.  Multiple cellular antigen detection by ICP-MS.

Authors:  O Ornatsky; V I Baranov; D R Bandura; S D Tanner; J Dick
Journal:  J Immunol Methods       Date:  2005-11-21       Impact factor: 2.303

3.  Pillars Article: Control of Regulatory T Cell Development by the Transcription Factor Foxp3. Science 2003. 299: 1057-1061.

Authors:  Shohei Hori; Takashi Nomura; Shimon Sakaguchi
Journal:  J Immunol       Date:  2017-02-01       Impact factor: 5.422

Review 4.  Control of regulatory T-cell differentiation and function by T-cell receptor signalling and Foxp3 transcription factor complexes.

Authors:  Masahiro Ono
Journal:  Immunology       Date:  2020-03-09       Impact factor: 7.397

5.  Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum.

Authors:  Sean C Bendall; Erin F Simonds; Peng Qiu; El-ad D Amir; Peter O Krutzik; Rachel Finck; Robert V Bruggner; Rachel Melamed; Angelica Trejo; Olga I Ornatsky; Robert S Balderas; Sylvia K Plevritis; Karen Sachs; Dana Pe'er; Scott D Tanner; Garry P Nolan
Journal:  Science       Date:  2011-05-06       Impact factor: 47.728

6.  Mass cytometry: technique for real time single cell multitarget immunoassay based on inductively coupled plasma time-of-flight mass spectrometry.

Authors:  Dmitry R Bandura; Vladimir I Baranov; Olga I Ornatsky; Alexei Antonov; Robert Kinach; Xudong Lou; Serguei Pavlov; Sergey Vorobiev; John E Dick; Scott D Tanner
Journal:  Anal Chem       Date:  2009-08-15       Impact factor: 6.986

7.  Two functional subsets of FOXP3+ regulatory T cells in human thymus and periphery.

Authors:  Tomoki Ito; Shino Hanabuchi; Yi-Hong Wang; Woong Ryeon Park; Kazuhiko Arima; Laura Bover; F Xiao-Feng Qin; Michel Gilliet; Yong-Jun Liu
Journal:  Immunity       Date:  2008-05-29       Impact factor: 31.745

Review 8.  Imbalance of regulatory T cells in human autoimmune diseases.

Authors:  Christian Dejaco; Christina Duftner; Beatrix Grubeck-Loebenstein; Michael Schirmer
Journal:  Immunology       Date:  2006-03       Impact factor: 7.397

9.  Functional delineation and differentiation dynamics of human CD4+ T cells expressing the FoxP3 transcription factor.

Authors:  Makoto Miyara; Yumiko Yoshioka; Akihiko Kitoh; Tomoko Shima; Kajsa Wing; Akira Niwa; Christophe Parizot; Cécile Taflin; Toshio Heike; Dominique Valeyre; Alexis Mathian; Tatsutoshi Nakahata; Tomoyuki Yamaguchi; Takashi Nomura; Masahiro Ono; Zahir Amoura; Guy Gorochov; Shimon Sakaguchi
Journal:  Immunity       Date:  2009-05-21       Impact factor: 31.745

10.  Defective regulatory and effector T cell functions in patients with FOXP3 mutations.

Authors:  Rosa Bacchetta; Laura Passerini; Eleonora Gambineri; Minyue Dai; Sarah E Allan; Lucia Perroni; Franca Dagna-Bricarelli; Claudia Sartirana; Susanne Matthes-Martin; Anita Lawitschka; Chiara Azzari; Steven F Ziegler; Megan K Levings; Maria Grazia Roncarolo
Journal:  J Clin Invest       Date:  2006-06       Impact factor: 14.808
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